MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 1200 . ANAEROMYXOBACTER-MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1200 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 10.00 ml - -
Solution B 1.00 ml - -
Solution C 0.20 ml - -
Solution D ("red mix") 0.20 ml - -
Solution E (electron acceptor) 0.05 ml - -
Solution F: Vitamin solution according to Wolin and coauthors 0.20 ml - -
1 Prepare solutions A, B, and D separately and anaerobically under 80% N2 + 20% CO2 and autoclave. Filter-sterilize solutions C, E and F and outgas with N2. After cooling, add about 1 ml solution B per 10 ml solution A, by this way adjusting pH to 7.2. Add 0.2 ml solution C, 0.2 ml solution D, 0.2 ml solution F and 0.05 ml solution E, each per 10 ml solution A.

Solution A #

904
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 1.0 g 1.106 18.929
MgCl2 x 6 H2O 0.5 g 0.553 2.721
KH2PO4 0.2 g 0.221 1.626
NH4Cl 0.3 g 0.332 6.204
KCl 0.3 g 0.332 4.451
CaCl2 x 2 H2O 15.0 mg 0.017 0.113
Na-acetate 0.4 g 0.442 5.394
Trace element solution SL-10 B 1.0 ml - -
Selenite-tungstate solution 2.0 ml - -
Resazurin
(1 g per L) 		1.0 ml - -
Distilled water 900.0 ml - -

Solution B #

50
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 2.5 g 50 595.188
Distilled water 50.0 ml - -

Solution C #

50
Compound Amount Unit Conc. [g/L] Conc. [mM]
DL-Dithiothreitol 385 mg 7.7 49.903
Distilled water 50 ml - -

Solution D ("red mix") #

50
Compound Amount Unit Conc. [g/L] Conc. [mM]
L-Cysteine 37.5 mg 0.75 6.19
Na2S x H2O
(dissolved separately in water) 		40.0 mg 0.8 10.251
Distilled water 50.0 ml - -

Solution E (electron acceptor) #

50
Compound Amount Unit Conc. [g/L] Conc. [mM]
Disodium fumarate 4 g 80 499.875
Distilled water 50 ml - -

Solution F: Vitamin solution according to Wolin and coauthors #

1000
Equipment needed: Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine-HCl x 2 H2O 5.0 mg 0.005 0.013
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
D-Calcium pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Trace element solution SL-10 B #

1008
Solution occurs in 1 other media (first defined in Medium 1200).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Distilled water 1000.0 ml - -
HCl
(25%) 		7.7 ml 1.925 52.798
FeSO4 x 7 H2O 1.5 g 1.488 5.353
ZnCl2 70.0 mg 0.069 0.509
MnCl2 x 4 H2O 100.0 mg 0.099 0.501
H3BO3 300.0 mg 0.298 4.813
CoCl2 x 6 H2O 190.0 mg 0.188 1.452
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.184
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.148

Selenite-tungstate solution #

1000
Solution occurs in 178 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.2
Equipment needed: Anaerobic workbench, Autoclave, Filter
Edit in Medium builder

Associated strains

All strains for this medium: 9

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 4.545 54.108
NaCl 1.006 17.208
MgCl2 x 6 H2O 0.503 2.473
Na-acetate 0.402 4.904
Disodium fumarate 0.364 2.272
Show more
CSV
JSON

Cultivation metadata from 9 strains BacDive

phyla 1 9 strains
oxygen anaerobe 9 strains
oxygen aerobe 5 strains
oxygen microaerophile 4 strains
temperature mesophilic 9 strains