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DSMZ

DSMZ 1200 . ANAEROMYXOBACTER-MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1200 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 10.00 ml - -
Solution B 1.00 ml - -
Solution C 0.20 ml - -
Solution D ("red mix") 0.20 ml - -
Solution E (electron acceptor) 0.05 ml - -
Solution F: Vitamin solution according to Wolin and coauthors 0.20 ml - -
1 Prepare solutions A, B, and D separately and anaerobically under 80% N2 + 20% CO2 and autoclave. Filter-sterilize solutions C, E and F and outgas with N2. After cooling, add about 1 ml solution B per 10 ml solution A, by this way adjusting pH to 7.2. Add 0.2 ml solution C, 0.2 ml solution D, 0.2 ml solution F and 0.05 ml solution E, each per 10 ml solution A.

Solution A #

904
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 1.0 g 1.106 18.929
MgCl2 x 6 H2O 0.5 g 0.553 2.721
KH2PO4 0.2 g 0.221 1.626
NH4Cl 0.3 g 0.332 6.204
KCl 0.3 g 0.332 4.451
CaCl2 x 2 H2O 15.0 mg 0.017 0.113
Na-acetate 0.4 g 0.442 5.394
Trace element solution SL-10 B 1.0 ml - -
Selenite-tungstate solution 2.0 ml - -
Resazurin
(1 g per L) 		1.0 ml - -
Distilled water 900.0 ml - -

Solution B #

50
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 2.5 g 50 595.188
Distilled water 50.0 ml - -

Solution C #

50
Compound Amount Unit Conc. [g/L] Conc. [mM]
DL-Dithiothreitol 385 mg 7.7 49.903
Distilled water 50 ml - -

Solution D ("red mix") #

50
Compound Amount Unit Conc. [g/L] Conc. [mM]
L-Cysteine 37.5 mg 0.75 6.19
Na2S x H2O
(dissolved separately in water) 		40.0 mg 0.8 10.251
Distilled water 50.0 ml - -

Solution E (electron acceptor) #

50
Compound Amount Unit Conc. [g/L] Conc. [mM]
Disodium fumarate 4 g 80 499.875
Distilled water 50 ml - -

Solution F: Vitamin solution according to Wolin and coauthors #

1000
Equipment needed: Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine-HCl x 2 H2O 5.0 mg 0.005 0.013
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
D-Calcium pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Trace element solution SL-10 B #

1008
Solution occurs in 1 other media (first defined in Medium 1200).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Distilled water 1000.0 ml - -
HCl
(25%) 		7.7 ml 1.925 52.798
FeSO4 x 7 H2O 1.5 g 1.488 5.353
ZnCl2 70.0 mg 0.069 0.509
MnCl2 x 4 H2O 100.0 mg 0.099 0.501
H3BO3 300.0 mg 0.298 4.813
CoCl2 x 6 H2O 190.0 mg 0.188 1.452
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.184
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.148

Selenite-tungstate solution #

1000
Solution occurs in 180 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.2
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 9

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 4.545 54.108
NaCl 1.006 17.208
MgCl2 x 6 H2O 0.503 2.473
Na-acetate 0.402 4.904
Disodium fumarate 0.364 2.272
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Cultivation metadata from 9 strains BacDive

phyla 1 9 strains
oxygen anaerobe 9 strains
oxygen aerobe 5 strains
oxygen microaerophile 4 strains
oxygen obligate aerobe 2 strains
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