11b .
MRS MEDIUM (pre-reduced)
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* Medium for Megasphaera sueciensis DSM 17042
Strictly anaerobic
Growth conditions: 30 °C
Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final pH:
6.2 - 6.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Casein peptone
(tryptic digest) |
10.00 | g | ||
|
|
Meat extract | 10.00 | g | ||
|
|
Yeast extract | 5.00 | g | ||
|
|
Glucose | 20.00 | g | ||
|
|
Tween 80 | 1.00 | g | ||
|
|
Resazurin | 1.00 | mg | ||
|
|
K2HPO4 | 2.00 | g | ||
|
|
Na-acetate | 5.00 | g | ||
|
|
(NH4)3 citrate | 2.00 | g | ||
|
|
MgSO4 x 7 H2O | 0.20 | g | ||
|
|
MnSO4 x H2O | 0.05 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
|
|
L-Cysteine HCl x H2O | 0.50 | g | ||
| 1 Cysteine is added after the medium has been boiled and cooled under CO2. Adjust pH to 6.2 - 6.5, distribute under N2 and autoclave. | |||||