11a .
MODIFIED MEDIUM 11
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Equipment needed:
Autoclave
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Medium 11 | 990 | ml | ||
|
|
Vitamin solution | 10 | ml | ||
| 1 Prepare medium 11 with additional vitamins. Adjust pH to 6.2 - 6.5 | |||||
Main sol. 11 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Casein peptone
(tryptic digest) |
10.00 | g | ||
|
|
Meat extract | 10.00 | g | ||
|
|
Yeast extract | 5.00 | g | ||
|
|
Glucose | 20.00 | g | ||
|
|
Tween 80 | 1.00 | g | ||
|
|
K2HPO4 | 2.00 | g | ||
|
|
Na-acetate | 5.00 | g | ||
|
|
(NH4)3 citrate | 2.00 | g | ||
|
|
MgSO4 x 7 H2O | 0.20 | g | ||
|
|
MnSO4 x H2O | 0.05 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 6.2 - 6.5. | |||||
Vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.00 | mg |
|
|
Folic acid | 2.00 | mg |
|
|
Pyridoxine hydrochloride | 10.00 | mg |
|
|
Thiamine-HCl x 2 H2O | 5.00 | mg |
|
|
Riboflavin | 5.00 | mg |
|
|
Nicotinic acid | 5.00 | mg |
|
|
Calcium pantothenate | 5.00 | mg |
|
|
p-Aminobenzoic acid | 1.00 | mg |
|
|
Vitamin B12 | 0.01 | mg |
|
|
Distilled water | 1000.00 | ml |