1189
1189 .
AEROBIC SULFOLOBALES MEDIUM WITHOUT SULFUR
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
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Medium for Aciditerrimonas ferrireducens DSM 45281
This strain uses the standard medium without modifications.
Growth conditions: 50 °C
Main sol. 1189 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Salt base solution | 1000.0 | ml | - | - | |
|
Yeast extract
(Difco) |
0.5 | g | 0.5 | - | |
| 1 Autoclave salt base solution and yeast extract (as a 10% solution) separately, and combine after cooling. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 1.30 | g | 1.3 | 9.838 | |
| KH2PO4 | 0.28 | g | 0.28 | 2.058 | |
| MgSO4 x 7 H2O | 0.25 | g | 0.25 | 1.014 | |
| CaCl2 x 2 H2O | 0.07 | g | 0.07 | 0.476 | |
| MnCl2 x 4 H2O | 1.80 | mg | 0.002 | 0.009 | |
| Na2B4O7 x 10 H2O | 4.50 | mg | 0.005 | - | |
| ZnSO4 x 7 H2O | 0.22 | g | 0.22 | 0.765 | |
| CuCl2 x 2 H2O | 0.05 | mg | 5.0e-5 | 2.9e-4 | |
| Na2MoO4 x 2 H2O | 0.03 | mg | 3.0e-5 | 1.2e-4 | |
| VOSO4 x n H2O | 0.03 | mg | 3.0e-5 | - | |
| CoSO4 x 7 H2O | 0.01 | mg | 1.0e-5 | 6.5e-5 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust to pH 2.5 with 10 N H2SO4. | |||||
Metadata
| Last modified: | 09.12.21 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Aciditerrimonas ferrireducens DSM 45281
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| (NH4)2SO4 | 1.3 | 9.838 |
| Yeast extract | 0.5 | - |
| KH2PO4 | 0.28 | 2.058 |
| MgSO4 x 7 H2O | 0.25 | 1.014 |
| ZnSO4 x 7 H2O | 0.22 | 0.765 |
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