Name: NSY-MEDIUM FOR POLYNUCLEOBACTER
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 1167 #

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Nutrient broth	1.0	g	1	-
Soy peptone (e.g. Bacto Soytone)	1.0	g	1	-
Yeast extract	1.0	g	1	-
Tap water	1000.0	ml	-	-
1 The following inorganic solution may substitute for tap water:
MgSO₄ x 7 H₂O	75.0	mg	0.075	0.304
Ca(NO₃)₂ x 4 H₂O	43.0	mg	0.043	0.182
NaHCO₃	16.0	mg	0.016	0.19
KCl	5.0	mg	0.005	0.067
K₂HPO₄ x 3 H₂O	3.7	mg	0.004	0.021
Distilled water	1000.0	ml	-	-
Trace element solution SL-4	0.1	ml	-	-
2 Adjust to pH 7.2.

Trace element solution SL-4 #

1000

Solution occurs in 23 other media (first defined in Medium 14).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Na₂-EDTA	0.50	g	0.5	1.487
FeSO₄ x 7 H₂O	0.20	g	0.2	0.719
ZnSO₄ x 7 H₂O	0.10	g	0.1	0.348
MnCl₂ x 4 H₂O	0.03	g	0.03	0.152
H₃BO₃	0.30	g	0.3	4.851
CoCl₂ x 6 H₂O	0.20	g	0.2	1.54
CuCl₂ x 2 H₂O	0.01	g	0.01	0.059
NiCl₂ x 6 H₂O	0.02	g	0.02	0.154
Na₂MoO₄ x 2 H₂O	0.03	g	0.03	0.124
Distilled water	1000.00	ml	-	-
1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.

All strains for this medium:	7
Strains with modifications:	1

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