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DSMZ

DSMZ 1145 . MS-MEDIUM (MODIFIED)

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Sulfurihydrogenibium rodmanii DSM 19533

* Modification: Final pH of the medium for DSM 19533 is pH 6.0-6.3.

Growth conditions: 12-24 h 68 °C

Main sol. 1145 #

ml
Equipment needed: Hungate tubes Anaerobic workbench Water bath
Compound Amount Unit Conc. [g/L] Conc. [mM]
Double distilled water
(anaerobic) 		800.00 ml - -
MS Buffer 200.00 ml - -
Elemental sulphur 5.00 g 5 -
NaS2O3 x 5 H2O 2.00 g 2 -
MgSO4 x 7 H2O 7.00 g 7 28.4
KCl 0.48 g 0.48 6.439
CaCl2 x 2 H2O 0.40 g 0.4 2.721
MgCl2 x 6 H2O 0.80 g 0.8 3.935
Solution A 20.00 ml - -
Solution B 1.50 ml - -
Solution C - Trace mineral solution (Ferguson and Mah, 1983) 10.00 ml - -
1 Each of the solutions (A, B, and C) is prepared with anaerobic double distilled water.
2 The medium is prepared anaerobically under nitrogen gas and dispensed into Hungate tubes or serum bottle sealed with rubber septa. The medium to gas phase ratio should be about 1:10 (v:v). Sterilise the medium by heating at 100° C for 3 hours on three consecutive days (e.g., in a water bath).
3 Add 4% O2 for microaerophilic conditions.

MS Buffer #

200
Equipment needed: Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 100 mM 4 100
1 100 mM NaOH saturated with CO2

Solution A #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 100 g 100 1869.51
MgCl2 x 6 H2O 100 g 100 491.877
CaCl2 x 2 H2O 40 g 40 272.081
Distilled water 1000 ml - -
1 Adjust to pH 4 with HCl

Solution B #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 x 3 H2O 200 g 200 1148.26
Distilled water 1000 ml - -

Solution C - Trace mineral solution (Ferguson and Mah, 1983) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA x 2 H2O 500 mg 0.5 1.487
Na2WO4 x 2 H2O 30 mg 0.03 0.091
CoCl2 x 6 H2O 150 mg 0.15 1.155
CuCl2 x 2 H2O 20 mg 0.02 0.117
MnCl2 x 4 H2O 100 mg 0.1 0.505
NiSO4 x 6 H2O 20 mg 0.02 0.129
FeSO4 x 7 H2O 100 mg 0.1 0.36
H2SeO3 10 mg 0.01 0.078
ZnCl2 100 mg 0.1 0.734
H3BO3 10 mg 0.01 0.162
AlCl3 x 6 H2O 40 mg 0.04 0.166
Na2MoO4 x 2 H2O 10 mg 0.01 0.041
Distilled water 1000 ml - -
1 Adjust pH to 3.0 with HCl

Metadata

Last modified: 15.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: * 6.0 - 6.3
Equipment needed: Anaerobic workbench, Hungate tubes, Water bath
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Gas composition

  • 4 % O2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 3
Strains with modifications: 3
Sulfurihydrogenibium rodmanii DSM 19533

  6.0 - 6.3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 7 28.4
Elemental sulphur 5 -
NH4Cl 2 37.39
MgCl2 x 6 H2O 2 9.838
NaS2O3 x 5 H2O 2 -
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Cultivation metadata from 3 strains BacDive

pH 4 - 6 3 strains
pH 6 - 8 3 strains
phyla 1 3 strains
nutrition chemolithotroph 1 strain
oxygen microaerophile 3 strains
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