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DSMZ

DSMZ 1137 . RUBRITALEA MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1137 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 1.5 g 1.5 -
Yeast extract 1.5 g 1.5 -
Glucose 1.5 g 1.5 8.326
Tris-HCl
(1M, pH 7.5) 		5.0 ml - 5
Hutner's salts 20.0 ml - -
Artificial sea water, 3 times concentrated 975.0 ml - -
1 Adjust to pH 7.5

Hutner's salts #

1000
Solution occurs in 2 other media (first defined in Medium 603).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA) 		10.000 g 10 52.318
MgSO4 x 7 H2O 29.700 g 29.7 120.499
CaCl2 x 2 H2O 3.335 g 3.335 22.685
(NH4)6Mo7O24 x 4 H2O 9.250 mg 0.009 -
FeSO4 x 7 H2O 99.000 mg 0.099 0.356
"Metals 44" 50.000 ml - -
Distilled water 950.000 ml - -
1 Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4.

"Metals 44" #

1000
Solution occurs in 34 other media (first defined in Medium 590).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-EDTA 250.0 mg 0.25 0.744
ZnSO4 x 7 H2O 1095.0 mg 1.095 3.808
FeSO4 x 7 H2O 500.0 mg 0.5 1.798
MnSO4 x H2O 154.0 mg 0.154 0.911
CuSO4 x 5 H2O 39.2 mg 0.039 0.157
Co(NO3)2 x 6 H2O 24.8 mg 0.025 0.085
Na2B4O7 x 10 H2O 17.7 mg 0.018 -
Distilled water 1000.0 ml - -
1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.

Artificial sea water, 3 times concentrated #

1000
Solution occurs in 1 other media (first defined in Medium 590).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 70.43 g 70.43 1205.17
Na2SO4 11.75 g 11.75 82.723
NaHCO3 2.88 g 2.88 34.283
KCl 1.99 g 1.99 26.694
KBr 0.29 g 0.29 2.437
MgCl2 x 6 H2O 31.86 g 31.86 156.712
CaCl2 x 2 H2O 4.35 g 4.35 29.589
H3BO3 0.08 g 0.08 1.294
Distilled water 1000.00 ml - -
1 Add NaHCO3 from a filter-sterilized stock solution when the medium has cooled.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 68.669 1175.04
MgCl2 x 6 H2O 31.064 152.794
Na2SO4 11.456 80.655
CaCl2 x 2 H2O 4.241 28.849
NaHCO3 2.808 33.426
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain