1134
1134 .
METHYLONATRUM MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1134 #
ml
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| K2HPO4 | 1 | g | 1 | 5.741 | |
| KNO3 | 1 | g | 1 | 9.891 | |
| Na2CO3 | 11 | g | 11 | 103.785 | |
| Methanol | 2 | ml | 1.584 | 49.435 | |
| NaCl | 18 | g | 18 | 308.008 | |
| MgSO4 x 7 H2O | 2 | ml | - | - | |
| NaS2O3 x 5 H2O | 18 | g | 18 | - | |
| Trace element solution SL-6 | 1 | ml | - | - | |
| Vitamin B12 | 100 | µg | 1.0e-4 | 7.4e-5 | |
| Distilled water | 1000 | ml | - | - | |
| 1 pH 10.0 | |||||
| 2 Prepare the medium without methanol, vitamin B12, MgSO4 x 7 H2O and NaS2O3 x 5 H2O in closed vessels with a medium to headspace ratio of 1:5 to 1:10. Add the methanol, MgSO4 x 7 H2O and NaS2O3 x 5 H2O from autoclaved stock solutions after sterilisation of the medium. Vitamin B12 is added from a filter sterilised stock solution to the sterile medium. Incubate the strains with shaking at 150 rpm. Growth is faster in the presence of 20 mM sodium acetate instead of methanol. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 |
| MnCl2 x 4 H2O | 0.03 | g | 0.03 | 0.152 |
| H3BO3 | 0.30 | g | 0.3 | 4.851 |
| CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 |
| CuCl2 x 2 H2O | 0.01 | g | 0.01 | 0.059 |
| NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 |
| Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Medium type: | Defined medium |
| Final pH: | 10.0 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |