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DSMZ

DSMZ 1132 . LC 2

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Methylosoma difficile DSM 18750

* Modification: Methanol or 20% methane and 2-5% CO2 in the headspace

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 20 °C

Main sol. 1132 #

ml
Equipment needed: Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 1.00 g 1 4.057
CaCl2 x 2 H2O 0.20 g 0.2 1.36
KNO3 0.05 g 0.05 0.495
Trace element solution SL-10 1.00 ml - -
H2O 1000.00 ml - -
1 Dispense 5 ml aliquots into Hungate tubes. After autoclaving add the following components per liter:
Phosphate buffer
(pH 6.8) 		2.00 ml - -
HEPES buffer
(pH 7.0, 2 M) 		1.00 ml 0.477 2
Fe(III)NH4-EDTA
(4mg/ml) 		1.00 ml - -
Methanol 0.30 ml 0.238 7.415
2 Adjust to pH 6.8.
3 Incubate in sealed containers. The strain held in the DSMZ has been cultivated on methanol, but the original publication indicates that the strain will also grow on methane.

Trace element solution SL-10 #

1000
Solution occurs in 266 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Phosphate buffer #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 3.6 g 36 253.594
KH2PO4 1.4 g 14 102.877
H2O 100.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.8
Equipment needed: Hungate tubes
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Gas composition

  • 20 % methane
  • 5 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2
Strains with modifications: 2
Methylosoma difficile DSM 18750

  20% methane, 5% CO2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 1 4.057
HEPES buffer 0.477 2
Methanol 0.238 7.415
CaCl2 x 2 H2O 0.2 1.36
Na2HPO4 0.072 0.507
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Cultivation metadata from 2 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 2 strains
oxygen microaerophile 1 strain
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