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DSMZ

DSMZ 1121 . MMJS MEDIUM (modified)

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1121 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.00 g 20 342.231
K2HPO4 0.09 g 0.09 0.517
KH2PO4 0.07 g 0.07 0.514
CaCl2 x 2 H2O 0.80 g 0.8 5.442
NH4Cl 1.25 g 1.25 23.369
MgSO4 x 7 H2O 4.00 g 4 16.229
MgCl2 x 6 H2O 3.00 g 3 14.756
KCl 0.33 g 0.33 4.427
Fe(III) citrate 0.01 g 0.01 0.041
FeSO4 x 7 H2O 0.01 g 0.01 0.036
Na2S2O3 x 5 H2O 3.00 g 3 12.088
Sulfur
(powdered) 		3.00 g 3 93.545
Trace mineral solution 10.00 ml - -
Distilled water 1000.00 ml - -
1 Finally, the mix gas (79%N2, 20%CO2, 1%O2) is compressed into gas phase (>80% volume of the tube or bottle) at 2 atm.
Vitamin solution 1.00 ml/l - -
NaHCO3 0.20 % 2 23.808
2 The pH of the medium is adjusted with NaOH to 6.8. Steam medium for 3 hours on each of 3 successive days. The separately autoclaved, concentrated solutions including each of follows are added to the medium. Then a mix gas (80%N2, 20%CO2) is purged for 5 min.

Trace mineral solution #

1000
Solution occurs in 1 other media (first defined in Medium 1121).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MnSO4 x n H2O 0.50 g 0.5 -
CoSO4 x 7 H2O 0.50 g 0.5 3.226
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 1.00 mg 1.0e-3 0.004
SrCl2 x 6 H2O 0.01 g 0.01 0.038
NaBr 0.01 g 0.01 0.097
KI 0.01 g 0.01 0.039
NiCl2 x 6 H2O 0.10 g 0.1 0.772
Na2SeO3 x 5 H2O 0.10 g 0.1 0.38
Fe2(SO4)3 x n H2O 1.00 g 1 2.501
H2WO4 0.10 g 0.1 0.4
Distilled water 1000.00 ml - -

Vitamin solution #

1000
Solution occurs in 17 other media (first defined in Medium 315).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine-HCl x 2 H2O 5.0 mg 0.005 0.013
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
D-Calcium pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.8
Equipment needed: Autoclave
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 20 342.231
MgSO4 x 7 H2O 4 16.229
Sulfur 3 93.545
MgCl2 x 6 H2O 3 14.756
Na2S2O3 x 5 H2O 3 12.088
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
oxygen microaerophile 1 strain
oxygen obligate aerobe 1 strain
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