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DSMZ

DSMZ 1116 . STEROIDOBACTER MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1116 #

ml
Equipment needed: Filter Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 1.00 g 1 17.112
MgCl2 x 6 H2O 0.40 g 0.4 1.968
KH2PO4 0.20 g 0.2 1.47
KCl 0.50 g 0.5 6.707
NH4Cl 0.25 g 0.25 4.674
CaCl2 x 2 H2O 0.15 g 0.15 1.02
Na2SO4 0.07 g 0.07 0.493
NaNO3 0.42 g 0.42 4.941
Distilled water 1000.00 ml - -
1 For growth with testosterone, dissolve testosterone in acetone (20 mg/ml), dispense portions of the solution, e.g. 0.1 ml portions for 10 ml medium, to anaerobic culture tubes. Let the solvent evaporate to dryness. Dispense 10 ml portions of medium and stream with N2/CO2 (80:20). Close the vessels and autoclave. Treat the vessels in an ultrasonic bath to detach and suspend the testosterone.
2 For growth with heptanoate, omit testosterone. Sterilize the gassed medium in anaerobic vessels by autoclaving. After cooling, add 325 mg/l heptanoic acid from a 20fold, pH adjusted, filter sterilized anaerobic stock solution.
3 After autoclaving, add (per 10 ml) 0.3 ml NaHCO3-solution (84 g/l, autoclaved under CO2-atmosphere), 1 µl trace element solution SL 10 (see below), 1 µl selenite tungstate solution (see below), and 1 µl vitamin solution (see below). Adjust pH to 7.2. Incubate the cultures in the dark for up to 4 weeks. Briefly shake the cultures once per day.

Trace element solution SL-10 #

1000
Solution occurs in 4 other media (first defined in Medium 1116).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%; 7.7 M) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -

Selenite-tungstate solution #

1000
Solution occurs in 178 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Vitamin solution #

1000
Solution occurs in 15 other media (first defined in Medium 461).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 50 mg 0.05 0.037
Pantothenic acid 50 mg 0.05 0.228
Riboflavin 50 mg 0.05 0.133
Pyridoxamine hydrochloride 10 mg 0.01 0.049
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Nicotinic acid 25 mg 0.025 0.203
Nicotine amide 25 mg 0.025 0.205
alpha-lipoic acid 50 mg 0.05 0.242
p-Aminobenzoic acid 50 mg 0.05 0.365
Thiamine-HCl x 2 H2O 50 mg 0.05 0.134
Distilled water 1000 ml - -
1 Stir for some hours, filter sterilize the solution.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.2
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 1 17.112
KCl 0.5 6.707
NaNO3 0.42 4.941
MgCl2 x 6 H2O 0.4 1.968
NH4Cl 0.25 4.674
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
phyla 1 1 strain
oxygen aerobe 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain