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DSMZ

DSMZ 1104 . DETHIOBACTER MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1104 #

ml
Equipment needed: Hungate tubes Water bath Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 6.00 g 5.976 102.26
K2HPO4 0.50 g 0.498 2.859
NaHCO3 8.00 g 7.968 94.851
Na2CO3 22.00 g 21.912 206.744
Sulfur
(powdered) 		1.50 g 1.494 46.586
NH4Cl 0.20 g 0.199 3.724
MgCl2 x 6 H2O 0.20 g 0.199 0.98
Trace elements solution (Pfennig & Lippert,1966) 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Yeast extract 0.05 g 0.05 -
Ethanol absolute 0.60 ml - -
Na-acetate 0.40 g 0.398 4.857
Na2S2O3 x 5 H2O 2.50 g 2.49 10.033
Wolin's vitamin solution (10x) 1.00 ml - -
Na2S x 9 H2O 0.24 g 0.239 0.995
Distilled water 1000.00 ml - -
1 Dissolve sodium chloride and hydrogenphosphate, then sparge solution with 100% N2 gas for 30 -45 min to make it anoxic. Add and dissolve carbonates, then dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials containing already the appropriate amount of sulfur, only to 30% of their volume to allow for a large headspace. Sterilize medium by heating cultivation vessels in a water bath to 90 - 100°C for 1- 2 hours on each of 3 successive days. After sterilization add ammonium chloride, magnesium chloride, trace elements, yeast extract, ethanol, acetate, thiosulfate, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The stock solutions of thiosulfate and vitamins should be sterilized by filtration. Adjust pH of the complete medium to 9.5.
2 After inoculation add sterile 100% H2 gas to 0.5 bar overpressure.

Trace elements solution (Pfennig & Lippert,1966) #

1000
Solution occurs in 28 other media (first defined in Medium 1369).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.20 g 2.2 7.913
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.03 g 0.03 0.485
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.03 g 0.03 0.176
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 pH 3.0-4.0

Selenite-tungstate solution #

1000
Solution occurs in 178 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Wolin's vitamin solution (10x) #

1000
Solution occurs in 305 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 27.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.5
Equipment needed: Filter, Gassing station, Hungate tubes, Water bath
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Gas composition

  • 66 % N2
  • 33 % H2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2CO3 21.912 206.744
NaHCO3 7.968 94.851
NaCl 5.976 102.26
Na2S2O3 x 5 H2O 2.49 10.033
Sulfur 1.494 46.586
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Cultivation metadata from 2 strains BacDive

phyla 2 2 strains
oxygen anaerobe 2 strains
temperature mesophilic 2 strains