This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Fe(III) citrate | 0.02 | g | 0.02 | 0.082 | |
CaCl2 x 2 H2O | 0.20 | g | 0.2 | 1.36 | |
MgSO4 x 7 H2O | 1.60 | g | 1.6 | 6.492 | |
KCl | 0.10 | g | 0.1 | 1.341 | |
NaCl | 10.00 | g | 10 | 171.116 | |
Na-caseinate | 0.50 | g | 0.5 | - | |
HEPES | 2.00 | g | 2 | 8.393 | |
NaHCO3 | 0.16 | g | 0.16 | 1.905 | |
H3BO3 | 20.00 | mg | 0.02 | 0.323 | |
KBr | 80.00 | mg | 0.08 | 0.672 | |
SrCl2 x 6 H2O | 30.00 | mg | 0.03 | 0.113 | |
Agar | 20.00 | g | 20 | 59.465 | |
Trace element solution SL-4 | 2.00 | ml | - | - | |
Distilled water | 998.00 | ml | - | - | |
Vitamin B12 | 0.50 | mg | 5.0e-4 | 3.7e-4 | |
1 Adjust to pH 7.2, check and adjust pH after autoclaving. Add vitamin B12 from a filter sterilized stock solution after autoclaving. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2-EDTA | 0.50 | g | 0.5 | 1.487 | |
FeSO4 x 7 H2O | 0.20 | g | 0.2 | 0.719 | |
ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
MnCl2 x 4 H2O | 0.03 | g | 0.03 | 0.152 | |
H3BO3 | 0.30 | g | 0.3 | 4.851 | |
CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 | |
CuCl2 x 2 H2O | 0.01 | g | 0.01 | 0.059 | |
NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 | |
Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 | |
Distilled water | 1000.00 | ml | - | - | |
1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. |
Last modified: | 24.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.2 |
Equipment needed: | Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Agar | 20 | 59.465 |
NaCl | 10 | 171.116 |
HEPES | 2 | 8.393 |
MgSO4 x 7 H2O | 1.6 | 6.492 |
Na-caseinate | 0.5 | - |
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