Name: MEDIUM FOR ENHYDROMYXA SALSUGINOSA
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 1102 #

Equipment needed: Filter

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Fe(III) citrate	0.02	g	0.02	0.082
CaCl₂ x 2 H₂O	0.20	g	0.2	1.36
MgSO₄ x 7 H₂O	1.60	g	1.6	6.492
KCl	0.10	g	0.1	1.341
NaCl	10.00	g	10	171.116
Na-caseinate	0.50	g	0.5	-
HEPES	2.00	g	2	8.393
NaHCO₃	0.16	g	0.16	1.905
H₃BO₃	20.00	mg	0.02	0.323
KBr	80.00	mg	0.08	0.672
SrCl2 x 6 H₂O	30.00	mg	0.03	0.113
Agar	20.00	g	20	59.465
Trace element solution SL-4	2.00	ml	-	-
Distilled water	998.00	ml	-	-
Vitamin B₁₂	0.50	mg	5.0e-4	3.7e-4
1 Adjust to pH 7.2, check and adjust pH after autoclaving. Add vitamin B₁₂ from a filter sterilized stock solution after autoclaving.

Trace element solution SL-4 #

1000

Solution occurs in 23 other media (first defined in Medium 14).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Na₂-EDTA	0.50	g	0.5	1.487
FeSO₄ x 7 H₂O	0.20	g	0.2	0.719
ZnSO₄ x 7 H₂O	0.10	g	0.1	0.348
MnCl₂ x 4 H₂O	0.03	g	0.03	0.152
H₃BO₃	0.30	g	0.3	4.851
CoCl₂ x 6 H₂O	0.20	g	0.2	1.54
CuCl₂ x 2 H₂O	0.01	g	0.01	0.059
NiCl₂ x 6 H₂O	0.02	g	0.02	0.154
Na₂MoO₄ x 2 H₂O	0.03	g	0.03	0.124
Distilled water	1000.00	ml	-	-
1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.

All strains for this medium:

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