DSMZ 108 . ACIDIPHILIUM ACIDOPHILUM MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 3.00 g 3 22.703
KH2PO4 0.50 g 0.5 3.674
MgSO4 x 7 H2O 1.00 g 1 4.057
KCl 0.10 g 0.1 1.341
Ca(NO3)2 x 4 H2O 18.00 mg 0.018 0.076
FeSO4 x 7 H2O
(0.1% w/v in 0.1 N H2SO4)
0.01 ml 1.0e-5 3.6e-5
Agar , for solid medium
optional
15.00 g 15 44.599
D-Glucose 10.00 g 10 55.506
Distilled water 1000.00 ml - -
1 In liquid medium, the pH should be adjusted to 3.5 with H2SO4. In solid medium, the pH should be adjusted to 4.5 with H2SO4 after autoclaving the medium. Sterilize the glucose separately and add to the sterile medium.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 4.5
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
D-Glucose 10 55.506
(NH4)2SO4 3 22.703
MgSO4 x 7 H2O 1 4.057
KH2PO4 0.5 3.674
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain