1076a .
SP4 MEDIUM WITH GLUCOSE
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Equipment needed:
Autoclave
Filter
Final pH:
7.4
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Medium 1076 | 517.0 | ml | ||
|
|
Glucose
(10 %) |
5.2 | ml | ||
| 1 Use medium 1076, but add 5.2 ml 10% glucose (filter-sterilized). | |||||
Main sol. 1076 #
515
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Tryptone | 5.00 | g | ||
|
|
Peptone | 2.65 | g | ||
|
|
PPLO broth | 1.75 | g | ||
|
|
Distilled water | 375.00 | ml | ||
| 1 Adjust pH to 7.4; autoclave at 121°C for 15 minutes. | |||||
| 2 Add aseptically: | |||||
|
|
Fetal bovine serum
(heat-inactivated) |
90.00 | ml | ||
|
|
CMRL 1066
(10x concentrated; GIBCO) |
25.00 | ml | ||
|
|
Yeast extract
(20%, autoclaved) |
17.50 | ml | ||
|
|
Yeast extract
(20%, autoclaved) |
5.00 | ml | ||
|
|
Phenol red
(1%, filter-sterilized) |
1.00 | ml | ||
|
|
L-Glutamine
(200 mM; GIBCO) |
1.70 | ml | ||