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DSMZ

DSMZ 1069 . MODIFIED BIEBL AND PFENNIG'S MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Marichromatium sp. DSM 21808

* Modification: For DSM 21808 and 21809. Adjust pH of final medium to 7.5. Add the following component after autoclaving from sterile stock solutions. Supplement medium with 1 ml/l Vitamin B12 (2 mg/l filter sterilised), 3 ml/l Na2S x 9 H2O (1M), 10 ml/l NaHCO3 (10%, autoclaved in sealed, half full vessels under CO2), and 3 g/l Sodium pyruvate.

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 3-7 days anaerobic in light 28 °C

Main sol. 1069 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.50 g 0.5 3.674
CaCl2 x 2 H2O 0.15 g 0.15 1.02
MgSO4 x 7 H2O 2.00 g 2 8.114
NH4Cl 0.34 g 0.34 6.356
NaCl 20.00 g 20 342.231
Yeast extract 0.40 g 0.4 -
Ferric citrate
(0.1% w/v) 		5.00 ml 0.005 0.02
Trace element solution SL-7 1.00 ml - -
Sodium pyruvate* 3.00 g/l - -
Vitamin B12*
(2 mg/l filter sterilised) 		1.00 ml/l - -
Na2S x 9 H2O*
(1M) 		3.00 ml/l - -
NaHCO3*
(10%, autoclaved in sealed, half full vessels under CO2) 		10.00 ml/l - -
Distilled water 1000.00 ml - -
1 Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas.

Trace element solution SL-7 #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Distilled water 1000 ml - -
HCl
(25%) 		1 ml 0.25 6.857
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 60 mg 0.06 0.97
CoCl2 x 6 H2O 200 mg 0.2 1.54
CuCl2 x 2 H2O 20 mg 0.02 0.117
NiCl2 x 6 H2O 20 mg 0.02 0.154
Na2MoO4 x 2 H2O 40 mg 0.04 0.165

Metadata

Last modified: 26.03.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: * 7.5
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Associated strains

All strains for this medium: 1
Strains with modifications: 1
Marichromatium sp. DSM 21808

  7.5
  3 g/l Sodium pyruvate
  1 ml/l Vitamin B12
  3 ml/l Na2S x 9 H2O
  10 ml/l NaHCO3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 20 342.231
MgSO4 x 7 H2O 2 8.114
KH2PO4 0.5 3.674
Yeast extract 0.4 -
NH4Cl 0.34 6.356
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 6 strains
pH 8 - 10 3 strains
phyla 1 8 strains
oxygen anaerobe 7 strains
oxygen facultative aerobe 2 strains
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