1059
1059 .
GEOALKALIBACTER MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1059 #
ml
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.5 | g | 0.499 | 9.329 | |
| KCl | 0.2 | g | 0.2 | 2.677 | |
| MgCl2 x 6 H2O | 0.1 | g | 0.1 | 0.491 | |
| KH2PO4 | 0.2 | g | 0.2 | 1.467 | |
| NaCl | 1.0 | g | 0.998 | 17.077 | |
| Trace element solution SL-10 | 1.0 | ml | - | - | |
| Selenite-tungstate solution | 1.0 | ml | - | - | |
| Na2CO3 | 3.0 | g | 2.994 | 28.249 | |
| NaHCO3 | 10.0 | g | 9.98 | 118.8 | |
|
Sulfur
(powdered) |
10.0 | g | 9.98 | 311.195 | |
| Yeast extract | 0.1 | g | 0.1 | - | |
| Na-acetate | 2.5 | g | 2.495 | 30.414 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients (ecxept carbonate, hydrogencarbonate, sulfur, yeast extract and acetate), then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add solid carbonate and bicarbonate, adjust pH to 9.0 - 9.2, and dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials containing already the appropriate amount of sulfur. Sterilize medium by autoclaving at 110°C for 20 min. Add yeast extract and acetate from sterile anoxic stock solutions prepared under 100% N2 gas. | |||||
Solution occurs in 267
other media (first defined in Medium 320).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Solution occurs in 180
other media (first defined in Medium 385).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaOH | 0.5 | g | 0.5 | 12.501 |
| Na2SeO3 x 5 H2O | 3.0 | mg | 0.003 | 0.011 |
| Na2WO4 x 2 H2O | 4.0 | mg | 0.004 | 0.012 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 24.07.23 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 9.0 - 9.2 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Sulfur | 9.98 | 311.195 |
| NaHCO3 | 9.98 | 118.8 |
| Na2CO3 | 2.994 | 28.249 |
| Na-acetate | 2.495 | 30.414 |
| NaCl | 0.998 | 17.077 |
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