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DSMZ

DSMZ 1058d . THIOHALOMONAS DENITRIFICANS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1058d #

ml
Equipment needed: Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 120.00 g 119.76 2049.29
K2HPO4 1.50 g 1.497 8.595
NH4Cl 0.50 g 0.499 9.329
KNO3 1.00 g 0.998 9.871
Trace elements solution (Pfennig & Lippert,1966) 1.00 ml - -
CaCl2 x 2 H2O 0.05 g 0.05 0.339
MgSO4 x 7 H2O 0.50 g 0.499 2.025
Na2S2O3 x 5 H2O 5.00 g 4.99 20.106
NaHCO3 5.00 g 4.99 59.4
Seven vitamins solution 1.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve sodium chloride, potassium hydrogenphosphate, ammonium chloride and potassium nitrate, then sparge solution with 80% N2 and 20% CO2 gas mixture for at least 30 - 45 min to remove dissolved oxygen. Dispense solution under same gas atmosphere in vials suitable for anaerobic cultures (e.g. Balch-type tubes) to 50% volume, close vials with butyl rubber septa and autoclave. Add trace elements, calcium chloride, magnesium sulfate, thiosulfate, and vitamins from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Thiosulfate and vitamins should be sterilized by filtration. Adjust pH of the complete medium to 7.5 - 7.8 using a sterile anoxic stock solution of sodium carbonate (5% w/v) prepared under 80% N2 and 20% CO2 gas atmosphere.
2 Note: Use at least 10% (v/v) as inoculum.

Trace elements solution (Pfennig & Lippert,1966) #

1000
Solution occurs in 28 other media (first defined in Medium 1369).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.20 g 2.2 7.913
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.03 g 0.03 0.485
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.03 g 0.03 0.176
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 pH 3.0-4.0

Seven vitamins solution #

1000
Solution occurs in 78 other media (first defined in Medium 503).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
D-(+)-biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 1.459
Thiamine-HCl x 2 H2O 200 mg 0.2 0.536
Distilled water 1000 ml - -

Metadata

Last modified: 17.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.5 - 7.8
Equipment needed: Autoclave, Filter, Gassing station
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 3
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 119.76 2049.29
NaHCO3 4.99 59.4
Na2S2O3 x 5 H2O 4.99 20.106
K2HPO4 1.497 8.595
KNO3 0.998 9.871
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Cultivation metadata from 3 strains BacDive

pH 6 - 8 2 strains
pH 8 - 10 1 strain
phyla 1 3 strains
oxygen anaerobe 3 strains
temperature mesophilic 3 strains