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DSMZ

DSMZ 1058c . THIOHALORHABDUS DENITRIFICANS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1058c #

ml
Equipment needed: Autoclave Gassing station Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 180.00 g 179.641 3073.93
K2HPO4 1.50 g 1.497 8.595
NH4Cl 0.50 g 0.499 9.329
Trace elements solution (Pfennig, 1965) 1.00 ml - -
CaCl2 x 2 H2O 0.05 g 0.05 0.339
MgSO4 x 7 H2O 0.50 g 0.499 2.025
Na2S2O3 x 5 H2O 5.00 g 4.99 20.106
NaHCO3 5.00 g 4.99 59.4
Seven vitamins solution 1.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve sodium chloride, potassium hydrogenphosphate and ammonium chloride, then sparge solution with 80% N2 and 20% CO2 gas mixture for at least 30 - 45 min to remove dissolved oxygen and to saturate the solution with CO2. Dispense solution in vials suitable for anaerobic cultures (e.g. Balch-type tubes) to 30% volume under 80% N2 and 20% CO2 gas atmosphere. Close vials with butyl rubber septa and autoclave. Add trace elements, calcium chloride, magnesium sulfate, thiosulfate, and vitamins from sterile stock solutions prepared under 100% N2 gas and bicarbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Thiosulfate and vitamins are sterilized by filtration. Adjust pH of the medium to 7.5 - 7.8 using a sterile stock solution of sodium carbonate (5% w/v).
2 After inoculation, add sterile air (with hypodermic needle through the rubber closure) to a concentration of ca. 4% (v/v) O2 (e.g., add 7 ml of air to a Balch-type tube with a total volume of 27 ml).
3 Note: Use at least 10% (v/v) as inoculum.

Trace elements solution (Pfennig, 1965) #

1000
Solution occurs in 28 other media (first defined in Medium 1369).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.20 g 2.2 7.913
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.03 g 0.03 0.485
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.03 g 0.03 0.176
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 pH 3.0-4.0

Seven vitamins solution #

1000
Solution occurs in 77 other media (first defined in Medium 503).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
D-(+)-biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 1.459
Thiamine-HCl x 2 H2O 200 mg 0.2 0.536
Distilled water 1000 ml - -

Metadata

Last modified: 17.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.5 - 7.8
Equipment needed: Autoclave, Filter, Gassing station
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Gas composition

  • 77 % N2
  • 19 % CO2
  • 4 % O2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Media similar to medium 1058c

1058 THIOHALOPHILUS MEDIUM
1058e THIOHALOBACTER THIOCYANATICUS MEDIUM

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 179.641 3073.93
NaHCO3 4.99 59.4
Na2S2O3 x 5 H2O 4.99 20.106
K2HPO4 1.497 8.595
NH4Cl 0.499 9.329
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen facultative anaerobe 1 strain
oxygen microaerophile 1 strain
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