Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Trypticase peptone
(BD BBL) |
5.0 | g | ||
|
Meat peptone
(pepsin-digested) |
5.0 | g | ||
|
Yeast extract | 10.0 | g | ||
|
Salt solution | 40.0 | ml | ||
|
NaCl | 60.0 | g | ||
|
MgSO4 x 7 H2O | 5.0 | g | ||
|
Betaine x H2O | 6.0 | g | ||
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | ||
|
L-Cysteine HCl x H2O | 0.5 | g | ||
|
Distilled water | 960.0 | ml | ||
1 Dissolve ingredients (except cysteine), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Add cysteine and adjust pH to 7.3. Thereafter, dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Adjust pH of complete medium to 7.3 - 7.5. |
Compound | Amount | Unit | |
---|---|---|---|
|
CaCl2 x 2 H2O | 0.25 | g |
|
MgSO4 x 7 H2O | 0.50 | g |
|
K2HPO4 | 1.00 | g |
|
KH2PO4 | 1.00 | g |
|
NaHCO3 | 10.00 | g |
|
NaCl | 2.00 | g |
|
Distilled water | 1000.00 | ml |