104c
104c .
PY + X MEDIUM (N2/CO2)
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Clostridium felsineum DSM 7320
* Modification: Adjust pH of complete medium to 6.3.
Growth conditions: anaerobic
37 °C
Main sol. 104c #
ml
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Trypticase peptone
(BD BBL) |
5.0 | g | 5 | - | |
|
Meat peptone
(pepsin-digested) |
5.0 | g | 5 | - | |
| Yeast extract | 10.0 | g | 10 | - | |
| Salt solution | 40.0 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
| Na2CO3 | 1.0 | g | 1 | 9.435 | |
| D-Glucose | 5.0 | g | 5 | 27.753 | |
| Distilled water | 960.0 | ml | - | - | |
| 1 Dissolve ingredients (except cysteine, carbonate and glucose) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add cysteine, then dispense under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose from a sterile anoxic stock solution prepared under 100% N2 gas atmosphere and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust pH of complete medium to 7.0, if necessary. | |||||
Solution occurs in 9
other media (first defined in Medium 104).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| CaCl2 x 2 H2O | 0.25 | g | 0.25 | 1.701 |
| MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 |
| K2HPO4 | 1.00 | g | 1 | 5.741 |
| KH2PO4 | 1.00 | g | 1 | 7.348 |
| NaHCO3 | 10.00 | g | 10 | 119.038 |
| NaCl | 2.00 | g | 2 | 34.223 |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | * 6.3 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Yeast extract | 10 | - |
| D-Glucose | 5 | 27.753 |
| Trypticase peptone | 5 | - |
| Meat peptone | 5 | - |
| Na2CO3 | 1 | 9.435 |
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Cultivation metadata from 127 strains
| halophily | non-halophilic | 1 strain |
| pH | 6 - 8 | 11 strains |
| pH | 8 - 10 | 6 strains |
| pH | 4 - 6 | 5 strains |
| phyla | 5 | 125 strains |
| Show more | ||