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DSMZ

DSMZ 1020 . SULFURICURVUM (MBM) MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1020 #

ml
Equipment needed: Autoclave Hungate tubes Filter Gassing station Serum bottle
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaNO3 0.2 g 0.2 2.348
KH2PO4 0.2 g 0.2 1.467
NH4Cl 0.2 g 0.2 3.732
MgCl2 x 6 H2O 0.4 g 0.399 1.964
KCl 0.2 g 0.2 2.677
CaCl2 x 2 H2O 0.1 g 0.1 0.679
Trace element solution SL-4 2.0 ml - -
Na2S2O3 x 5 H2O 2.5 g 2.495 10.053
Distilled water 1000.0 ml - -
1 Dissolve ingredients, except sodium thiosulfate, then sparge solution with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Adjust pH to 7.0, then distribute medium under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials (e.g., 20 ml medium in 100 ml bottles) and autoclave. Prior to inoculation, add thiosulfate from an anoxic stock solution prepared under 100% N2 gas and sterilized by filtration. Adjust pH of complete medium to 7.0, if necessary.
2 After inoculation, pressurize culture vessels to 0.5 bar overpressure with sterile 100% H2 gas.

Trace element solution SL-4 #

1000
Solution occurs in 23 other media (first defined in Medium 14).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 0.50 g 0.5 1.487
FeSO4 x 7 H2O 0.20 g 0.2 0.719
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.

Metadata

Last modified: 17.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes, Serum bottle
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Gas composition

  • 53 % N2
  • 33 % H2
  • 13 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2S2O3 x 5 H2O 2.495 10.053
MgCl2 x 6 H2O 0.399 1.964
NH4Cl 0.2 3.732
KCl 0.2 2.677
NaNO3 0.2 2.348
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain