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DSMZ

DSMZ 1019 . ROUF'S MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1019 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 5.000 g 5 -
Peptone 5.000 g 5 -
MgSO4 x 7 H2O 0.200 g 0.2 0.811
CaCl2 x 6 H2O 0.050 g 0.05 0.228
Fe(NH4)citrate 0.015 g 0.015 -
MnSO4 x 4 H2O 0.050 g 0.05 0.224
FeCl3 x 4 H2O 0.010 g 0.01 0.062
Wolin's vitamin solution 10.000 ml - -
Trace elements solution 1.000 ml - -
Distilled water 1000.000 ml - -
Agar , for solid medium 17.000 g 17 50.546
1 pH 7.1 (adjusted with NaOH if needed)
2 The medium may be solifidied with 17 g/l agar.

Trace elements solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 12.80 g 12.8 66.967
CoCl2 x 6 H2O 0.17 g 0.17 1.309
CaCl2 x 2 H2O 0.10 g 0.1 0.68
FeSO4 x 7 H2O 0.10 g 0.1 0.36
MnCl2 x 4 H2O 0.10 g 0.1 0.505
NaCl 0.10 g 0.1 1.711
Na2MoO4 x 2 H2O 0.10 g 0.1 0.413
Distilled water 1000.00 ml - -
1 First dissolve the nitrilotriacetic acid and adjust the pH to 6.5 with KOH, then add the minerals. The final pH is 7.0, adjusted with KOH.

Wolin's vitamin solution #

1000
Solution occurs in 54 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 0.021
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.1
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 17 50.546
Yeast extract 5 -
Peptone 5 -
MgSO4 x 7 H2O 0.2 0.811
CaCl2 x 6 H2O 0.05 0.228
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Cultivation metadata from 2 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 2 strains
oxygen aerobe 1 strain
oxygen facultative anaerobe 1 strain
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