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DSMZ

DSMZ 1005 . THERMODESULFOBIUM MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Thermodesulfobium narugense DSM 14796

This strain uses the standard medium without modifications.

Growth conditions: 3-7 days anaerobic 50 °C

Main sol. 1005 #

ml
Equipment needed: Autoclave Filter Serum bottle Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.78 g 0.772 4.43
KH2PO4 0.75 g 0.742 5.451
Na2-EDTA 0.04 g 0.04 0.118
FeSO4 x 7 H2O 0.01 g 0.01 0.036
MgSO4 x 7 H2O 0.25 g 0.247 1.003
CaCl2 x 2 H2O 0.03 g 0.03 0.202
Trace element solution 10.00 ml - -
NaCl 0.20 g 0.198 3.385
NH4Cl 0.50 g 0.495 9.246
Na2SO4 2.80 g 2.77 19.498
Na-acetate 0.15 g 0.148 1.809
Wolin's vitamin solution (10x) 1.00 ml - -
L-Cysteine HCl x H2O 0.25 g 0.247 1.408
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except vitamins and cysteine), adjust pH to 5.5 with 10 N H2SO4 and sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials (e.g., 20 ml of medium in 50 ml bottles) and autoclave. Prior to inoculation add vitamins (sterilized by filtration) and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of complete medium to 5.5 - 6.0, if necessary.
2 After inoculation pressurize vials to 0.5 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.

Trace element solution #

1000
Solution occurs in 14 other media (first defined in Medium 318).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA) 		12.80 g 12.8 66.967
FeCl2 x 4 H2O 1.00 g 1 5.03
MnCl2 x 4 H2O 0.10 g 0.1 0.505
CoCl2 x 6 H2O 0.03 g 0.03 0.231
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnCl2 0.10 g 0.1 0.734
CuCl2 0.02 g 0.02 0.149
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
NiCl2 x 6 H2O 0.10 g 0.1 0.772
NaCl 1.00 g 1 17.112
Na2SeO3 x 5 H2O 0.03 g 0.03 0.114
Na2WO4 x 2 H2O 0.04 g 0.04 0.121
Distilled water 1000.00 ml - -
1 First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. Finally adjust pH to 6.5 with KOH and make up to 1000.00 ml.

Wolin's vitamin solution (10x) #

1000
Solution occurs in 306 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 17.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 5.5 - 6.0
Equipment needed: Autoclave, Filter, Gassing station, Serum bottle
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Gas composition

  • 80 % H2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1
Thermodesulfobium narugense DSM 14796

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2SO4 2.77 19.498
K2HPO4 0.772 4.43
KH2PO4 0.742 5.451
NH4Cl 0.495 9.246
L-Cysteine HCl x H2O 0.247 1.408
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain