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DSMZ

DSMZ 1003 . HYDROGEN-OXYDIZING MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Sulfurihydrogenibium azorense DSM 15241

This strain uses the standard medium without modifications.

Growth conditions: 3-7 days 70 °C

Main sol. 1003 #

ml
Equipment needed: Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
Water 1000.00 ml - -
1 Place 1000 ml of anaerobic water (CO2 water) in a flask. Add the following compounds:
MgSO4 x 7 H2O 7.00 g 7 28.4
NaS2O3 2.00 g 2 -
CaCl2 x 2 H2O 0.40 g 0.4 2.721
KCl 0.48 g 0.48 6.439
MgCl2 0.78 g 0.78 8.192
MES 1.95 g 1.95 9.988
2 Add solutions A, B, and Trace mineral solution (see recipes below):
Solution A (100x solution) 2.00 ml - -
Solution B (500x solution) 1.50 ml - -
Trace mineral solution (100x) 10.00 ml - -
3 pH the media to 6.0 and gas the flask containing the media with CO2 for 20 min at least. Gas first culture tube in rack and place 5 ml of media in previous tube. Place stopper on loaded culture tube, crimp tube cap onto stopper. Autoclave the medium for 20 min., 121°C. Add 1 ml of O2 to each tube before inoculation. After the inoculation the tubes are pressurized with H2 (138 KP).

Solution A (100x solution) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 100 g 100 1869.51
MgCl2 x H2O 100 g 100 883.189
CaCl2 x 2 H2O 40 g 40 272.081
Distilled water 1000 ml - -
1 Adjust the pH to 4 with HCl.

Solution B (500x solution) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 x 3 H2O 200 g 200 1148.26
Distilled water 1000 ml - -

Trace mineral solution (100x) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-EDTA x 2 H2O 500 mg 0.5 1.487
CoCl2 x 6 H2O 150 mg 0.15 1.155
MnCl2 x 4 H2O 100 mg 0.1 0.505
FeSO4 x 7 H2O 100 mg 0.1 0.36
ZnCl2 100 mg 0.1 0.734
AlCl3 x 6 H2O 40 mg 0.04 0.166
Na-tungstate x 2 H2O 30 mg 0.03 -
CuCl 20 mg 0.02 0.202
NiSO4 x 6 H2O 20 mg 0.02 0.129
Se-acid 10 mg 0.01 0.069
HBO3 10 mg 0.01 0.167
Na2MoO4 x 2 H2O 10 mg 0.01 0.041
Distilled water 1000 ml - -
1 Adjust the pH of the solution to 3.0 with HCl.

Metadata

Last modified: 15.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Anaerobic workbench, Autoclave
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Associated strains

All strains for this medium: 1
Sulfurihydrogenibium azorense DSM 15241

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 7 28.4
NaS2O3 2 -
MES 1.95 9.988
MgCl2 0.78 8.192
KCl 0.48 6.439
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
temperature thermophilic 1 strain