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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.



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Current criteria: Na2CO3

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14 CLOSTRIDIUM FORMICACETICUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar
optional
14.837 -
K2HPO4 9.891 56.789
D-Fructose 4.946 27.451
Yeast extract 4.946 -
Na2CO3 0.989 9.332
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Solutions:
  • 10 ml Trace element solution SL-4
Gas phase:
  • 100 % N2

31 ALKALINE NUTRIENT AGAR

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Na2CO3 anhydrous 5.3 50.006
Peptone 5 -
NaHCO3 4.2 49.996
Meat extract 3 -
Distilled water - -
Solutions:
  • 100 ml Na-sesquicarbonate solution

52 CLOSTRIDIUM KLUYVERI MEDIUM

Compound Conc. [g/L] Conc. [mM]
K-acetate 10 101.893
Na2CO3 1 9.435
Yeast extract 1 -
K2HPO4 0.31 1.78
NH4Cl 0.25 4.674
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Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 1 ml Seven vitamins solution
Gas phase:
  • 80 % N2
  • 20 % CO2

60 MOORELLA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Glucose 17.946 99.612
K2HPO4 6.979 40.069
Yeast extract 4.985 -
Tryptone 4.985 -
KH2PO4 4.487 32.969
Na2CO3 1.994 18.814
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Solutions:
  • 843 ml Solution A
  • 50 ml Solution B
  • 50 ml Solution C
  • 40 ml Solution D
  • 10 ml Solution E
  • 10 ml Solution F
Gas phase:
  • 100 % CO2

76 GOTTSCHALKIA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Uric acid 2 11.897
Na2CO3 1.5 14.153
Yeast extract 1 -
K2HPO4 0.91 5.225
KOH 0.67 11.942
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Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
Gas phase:
  • 100 % N2

98 RHIZOBIUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
air-dried garden soil 80 -
Agar 15 44.599
Mannitol 10 54.894
Yeast extract 1 -
Na2CO3 0.2 1.887
Distilled water - -
Solutions:
  • 200 ml Soil extract

104c PY + X MEDIUM (N2/CO2)

Compound Conc. [g/L] Conc. [mM]
Yeast extract 10 -
D-Glucose 5 27.753
Trypticase peptone 5 -
Meat peptone 5 -
Na2CO3 1 9.435
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Solutions:
  • 40 ml Salt solution
Gas phase:
  • 80 % N2
  • 20 % CO2

110a CMC MEDIUM (N2/CO2)

Compound Conc. [g/L] Conc. [mM]
Ground beef 495.049 -
Casitone 29.703 -
K2HPO4 4.951 28.422
Yeast extract 4.951 -
D-Glucose 3.96 21.983
Na2CO3 1.485 14.012
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Solutions:
  • 1000 ml Meat filtrate
  • 10 ml Sugar mix
Gas phase:
  • 80 % N2
  • 20 % CO2

113 THIOBACILLUS DENITRIFICANS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na2S2O3 x 5 H2O 4.985 20.086
KNO3 1.994 19.723
KH2PO4 1.994 14.653
Na2CO3 0.997 9.407
NH4Cl 0.997 18.639
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Solutions:
  • 962 ml Solution A
  • 20 ml Solution B
  • 20 ml Solution C
  • 1 ml Solution D
  • 2 ml Trace element solution SL-4
Gas phase:
  • 100 % N2

113a SULFURIMONAS HONGKONGENSIS MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 24.9 426.085
Na2S2O3 x 5 H2O 4.98 20.066
KNO3 1.992 19.703
KH2PO4 1.992 14.638
Na2CO3 0.996 9.397
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Solutions:
  • 942 ml Solution A
  • 40 ml Solution B
  • 20 ml Solution C
  • 1 ml Solution D
  • 1 ml Solution E
  • 2 ml Trace element solution SL-4
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 100 % N2
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