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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: Na2-EDTA

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14 CLOSTRIDIUM FORMICACETICUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar
optional 		14.837 -
K2HPO4 9.891 56.789
D-Fructose 4.946 27.451
Yeast extract 4.946 -
Na2CO3 0.989 9.332
Na-thioglycolate 0.742 6.502
Na2-EDTA 0.005 0.015
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Solutions:
  • 10 ml Trace element solution SL-4
Gas phase:
  • 100 % N2

28 PFENNIG'S MEDIUM I

Compound Conc. [g/L] Conc. [mM]
NaHCO3 1.5 17.856
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.5 2.029
Ammonium chloride 0.35 6.543
KCl 0.35 4.695
KH2PO4 0.35 2.572
Ammonium acetate 0.25 3.243
Pyruvic acid sodium salt 0.25 2.272
CaCl2 x 2 H2O 0.25 1.701
Dextrose 0.25 1.262
Yeast extract 0.25 -
Na2-EDTA 0.003 0.009
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Solutions:
  • 460 ml Solution A
  • 40 ml Sulfide solution, 1.5%
  • 50 ml Bicarbonate solution
  • 450 ml Resazurin solution
  • 26 ml Pfennig's heterotrophic salts solution
  • 1 ml Vitamin B12 solution
  • 10 ml Neutralized sulfide solution 3% (w/v)
  • 1 ml Trace element solution SL-12 B

28a THIORHODOCOCCUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 20 342.231
NaHCO3 1.5 17.856
MgCl2 x 6 H2O 1 4.919
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.5 2.029
Sodium Thiosulfate 0.5 3.162
Ammonium chloride 0.35 6.543
KCl 0.35 4.695
KH2PO4 0.35 2.572
Ammonium acetate 0.25 3.243
Pyruvic acid sodium salt 0.25 2.272
CaCl2 x 2 H2O 0.25 1.701
Dextrose 0.25 1.262
Yeast extract 0.25 -
Na2-EDTA 0.003 0.009
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Solutions:
  • 460 ml Solution A
  • 40 ml Sulfide solution, 1.5%
  • 50 ml Bicarbonate solution
  • 450 ml Resazurin solution
  • 26 ml Pfennig's heterotrophic salts solution
  • 1 ml Vitamin B12 solution
  • 10 ml Neutralized sulfide solution 3% (w/v)

28b THIORHODOCOCCUS MEDIUM II

Compound Conc. [g/L] Conc. [mM]
NaCl 15 256.674
MgCl2 x 6 H2O 2.5 12.297
NaHCO3 1.5 17.856
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.5 2.029
Na2S2O3 x 5 H2O 0.5 2.015
Ammonium chloride 0.35 6.543
KCl 0.35 4.695
KH2PO4 0.35 2.572
Ammonium acetate 0.25 3.243
Pyruvic acid sodium salt 0.25 2.272
CaCl2 x 2 H2O 0.25 1.701
Dextrose 0.25 1.262
Yeast extract 0.25 -
Na2-EDTA 0.003 0.009
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Solutions:
  • 460 ml Solution A
  • 40 ml Sulfide solution, 1.5%
  • 50 ml Bicarbonate solution
  • 450 ml Resazurin solution
  • 26 ml Pfennig's heterotrophic salts solution
  • 1 ml Vitamin B12 solution
  • 10 ml Neutralized sulfide solution 3% (w/v)

39 PFENNIG'S MEDIUM I WITH YEAST EXTRACT

Compound Conc. [g/L] Conc. [mM]
NaHCO3 1.5 17.856
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.5 2.029
Yeast extract 0.5 -
Ammonium chloride 0.35 6.543
KCl 0.35 4.695
KH2PO4 0.35 2.572
Ammonium acetate 0.25 3.243
Pyruvic acid sodium salt 0.25 2.272
CaCl2 x 2 H2O 0.25 1.701
Dextrose 0.25 1.262
Na2-EDTA 0.003 0.009
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Solutions:
  • 460 ml Solution A
  • 40 ml Sulfide solution, 1.5%
  • 50 ml Bicarbonate solution
  • 450 ml Resazurin solution
  • 26 ml Pfennig's heterotrophic salts solution
  • 1 ml Vitamin B12 solution
  • 10 ml Neutralized sulfide solution 3% (w/v)
  • 1 ml Trace element solution SL-12 B

43 PFENNIG'S MEDIUM I WITH SALT

Compound Conc. [g/L] Conc. [mM]
NaCl 30 513.347
NaHCO3 1.5 17.856
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.5 2.029
Ammonium chloride 0.35 6.543
KCl 0.35 4.695
KH2PO4 0.35 2.572
Ammonium acetate 0.25 3.243
Pyruvic acid sodium salt 0.25 2.272
CaCl2 x 2 H2O 0.25 1.701
Dextrose 0.25 1.262
Yeast extract 0.25 -
Na2-EDTA 0.003 0.009
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Solutions:
  • 460 ml Solution A
  • 40 ml Sulfide solution, 1.5%
  • 50 ml Bicarbonate solution
  • 450 ml Resazurin solution
  • 26 ml Pfennig's heterotrophic salts solution
  • 1 ml Vitamin B12 solution
  • 10 ml Neutralized sulfide solution 3% (w/v)
  • 1 ml Trace element solution SL-12 B

46 PFENNIG'S MEDIUM I WITH SALT

Compound Conc. [g/L] Conc. [mM]
NaCl 10 171.116
NaHCO3 1.5 17.856
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.5 2.029
Ammonium chloride 0.35 6.543
KCl 0.35 4.695
KH2PO4 0.35 2.572
Ammonium acetate 0.25 3.243
Pyruvic acid sodium salt 0.25 2.272
CaCl2 x 2 H2O 0.25 1.701
Dextrose 0.25 1.262
Yeast extract 0.25 -
Na2-EDTA 0.003 0.009
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Solutions:
  • 460 ml Solution A
  • 40 ml Sulfide solution, 1.5%
  • 50 ml Bicarbonate solution
  • 450 ml Resazurin solution
  • 26 ml Pfennig's heterotrophic salts solution
  • 1 ml Vitamin B12 solution
  • 10 ml Neutralized sulfide solution 3% (w/v)
  • 1 ml Trace element solution SL-12 B

68 HALOTHIOBACILLUS NEAPOLITANUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar
optional 		15 44.599
Na2S2O3 x 5 H2O 10 40.292
KH2PO4 4 29.393
K2HPO4 4 22.965
MgSO4 x 7 H2O 0.8 3.246
NH4Cl 0.4 7.478
Na2-EDTA 0.25 0.744
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Solutions:
  • 5 ml Trace element solution (Vishniac & Santer, 1957)

69 STARKEYA NOVELLA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar
optional 		14.892 -
Na2HPO4 x 12 H2O 10.524 74.133
Na2S2O3 x 5 H2O 4.975 20.046
KH2PO4 1.489 10.943
NH4Cl 0.298 5.568
Yeast extract 0.298 -
Na2-EDTA 0.249 0.74
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Solutions:
  • 900 ml Solution A
  • 50 ml Solution B
  • 5 ml Solution C
  • 50 ml Solution D
  • 5 ml Trace element solution (Vishniac & Santer, 1957)

95 DESULFUROMONAS ACETOXIDANS MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 19.92 340.868
Na2-DL-malate 2.649 14.88
MgCl2 x 6 H2O 1.992 9.798
NaHCO3 1.843 21.934
Na2SO4 1.763 12.412
KH2PO4 0.996 7.319
MgSO4 x 7 H2O 0.996 4.041
Yeast extract 0.498 -
Na2S x 9 H2O 0.299 1.244
NH4Cl 0.299 5.586
CaCl2 x 2 H2O 0.1 0.677
Na2-EDTA x 2 H2O 0.005 0.015
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Solutions:
  • 951 ml Solution A
  • 3 ml Solution B
  • 40 ml Solution C
  • 10 ml Solution D
  • 1 ml Trace element solution SL-11
Gas phase:
  • 100 % N2
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