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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.



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Current criteria: KH2PO4

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3 AZOTOBACTER MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
CaCO3 5 49.955
Glucose 5 27.753
Mannitol 5 27.447
K2HPO4 0.9 5.167
KH2PO4 0.1 0.735
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Gas phase:
  • 80 % N2
  • 20 % CO2

6 ALLANTOIN MINERAL MEDIUM

Compound Conc. [g/L] Conc. [mM]
Allantoin 20 126.489
Agar 15 -
K2HPO4 0.8 4.593
MgSO4 x 7 H2O 0.5 2.029
KH2PO4 0.2 1.47
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13 BACILLUS ACIDOCALDARIUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar 20 59.466
Glucose 1 5.551
Yeast extract 1 -
KH2PO4 0.6 4.409
MgSO4 x 7 H2O 0.5 2.029
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Solutions:
  • 500 ml Solution A
  • 500 ml Solution B

25 RHODOPSEUDOMONAS GLOBIFORMIS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Mannitol 1.5 8.234
KH2PO4 0.5 3.674
Na-gluconate 0.5 2.292
NH4Cl 0.4 7.478
NaCl 0.4 6.845
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Solutions:
  • 1 ml Trace element solution SL-6

26 ACID RHODOSPIRILLACEAE MEDIUM

Compound Conc. [g/L] Conc. [mM]
Disodium succinate 1 6.171
Ammonium acetate 0.5 6.487
KH2PO4 0.5 3.674
NH4Cl 0.4 7.478
NaCl 0.4 6.845
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Solutions:
  • 1 ml Trace element solution SL-6
Gas phase:
  • 100 % N2

27 RHODOSPIRILLACEAE MEDIUM (modified)

Compound Conc. [g/L] Conc. [mM]
Disodium succinate 1 6.171
Ammonium acetate 0.5 6.487
KH2PO4 0.5 3.674
NH4Cl 0.4 7.478
NaCl 0.4 6.845
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Solutions:
  • 1 ml Trace element solution SL-6

28 PFENNIG'S MEDIUM I

Compound Conc. [g/L] Conc. [mM]
NaHCO3 1.5 17.856
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.5 2.029
Ammonium chloride 0.35 6.543
KCl 0.35 4.695
KH2PO4 0.35 2.572
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Solutions:
  • 460 ml Solution A
  • 40 ml Sulfide solution, 1.5%
  • 50 ml Bicarbonate solution
  • 450 ml Resazurin solution
  • 26 ml Pfennig's heterotrophic salts solution
  • 1 ml Vitamin B12 solution
  • 10 ml Neutralized sulfide solution 3% (w/v)
  • 1 ml Trace element solution SL-12 B

28a THIORHODOCOCCUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 20 342.231
NaHCO3 1.5 17.856
MgCl2 x 6 H2O 1 4.919
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.5 2.029
Sodium Thiosulfate 0.5 3.162
Ammonium chloride 0.35 6.543
KCl 0.35 4.695
KH2PO4 0.35 2.572
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Solutions:
  • 460 ml Solution A
  • 40 ml Sulfide solution, 1.5%
  • 50 ml Bicarbonate solution
  • 450 ml Resazurin solution
  • 26 ml Pfennig's heterotrophic salts solution
  • 1 ml Vitamin B12 solution
  • 10 ml Neutralized sulfide solution 3% (w/v)

28b THIORHODOCOCCUS MEDIUM II

Compound Conc. [g/L] Conc. [mM]
NaCl 15 256.674
MgCl2 x 6 H2O 2.5 12.297
NaHCO3 1.5 17.856
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.5 2.029
Na2S2O3 x 5 H2O 0.5 2.015
Ammonium chloride 0.35 6.543
KCl 0.35 4.695
KH2PO4 0.35 2.572
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Solutions:
  • 460 ml Solution A
  • 40 ml Sulfide solution, 1.5%
  • 50 ml Bicarbonate solution
  • 450 ml Resazurin solution
  • 26 ml Pfennig's heterotrophic salts solution
  • 1 ml Vitamin B12 solution
  • 10 ml Neutralized sulfide solution 3% (w/v)

29 PFENNIG'S MEDIUM II

Compound Conc. [g/L] Conc. [mM]
NaHCO3 1.5 17.856
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.52 2.11
Ammonium chloride 0.364 6.805
KCl 0.364 4.883
KH2PO4 0.364 2.675
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Solutions:
  • 460 ml Solution A
  • 40 ml Solution B
  • 50 ml Solution C
  • 450 ml Solution D
  • 26 ml Solution E
  • 10 ml Solution F
  • 1 ml Trace element solution SL-10 B
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