{
    "id": 523,
    "name": "Main sol. 257",
    "volume": 1000,
    "recipe": [
        {
            "recipe No.": 1,
            "compound": "Yeast extract",
            "compound_id": 16,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": "BD Bacto",
            "amount": 3,
            "unit": "g",
            "g_l": 3,
            "mmol_l": null,
            "optional": "no"
        },
        {
            "recipe No.": 2,
            "compound": "Peptone",
            "compound_id": 1,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": "BD Bacto",
            "amount": 0.6,
            "unit": "g",
            "g_l": 0.6,
            "mmol_l": null,
            "optional": "no"
        },
        {
            "recipe No.": 4,
            "compound": "Tris-HCl buffer",
            "compound_id": 285,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": "0.05 M, pH 7.5",
            "amount": 1000,
            "unit": "ml",
            "g_l": null,
            "mmol_l": 50,
            "optional": "no"
        }
    ],
    "steps": [
        {
            "recipe No.": null,
            "step": "Preparation of double layered agar plates:<p>\r\n<b>Bottom layer:</b> Dissolve 12.0 g Bacto agar in 900 ml distilled water and autoclave. After cooling to 60\u00b0C add 100 ml of sterile YP medium (autoclaved at 121\u00b0C for 15 min) and pour plates. Dry plates at room temperature for 2 - 3 days .<p>\r\n<b>Top layer:</b> Grow prey bacterium on agar slants using medium 54. Prepare 5 ml aliquots of 0.6% Bacto agar in distilled water and autoclave in test tubes. After cooling to 45\u00b0C in a water bath add 0.5 ml sterile YP medium (autoclaved at 121\u00b0C for 15 min) and 0.5 ml cell suspension of host organism, mix and pour top layer. For the top layer of one plate use one 24 h old agar slant culture and wash off grown cells in 0.5 ml sterile water.<p>"
        },
        {
            "recipe No.": null,
            "step": "Inoculate double layer plates the same day when prepared."
        }
    ],
    "equipment": {
        "2004": "Autoclave",
        "5151": "Water bath"
    },
    "Sol. No.": null,
    "main solution": "no"
}