{
    "id": 4390,
    "name": " Solution A",
    "volume": 902,
    "recipe": [
        {
            "recipe No.": 1,
            "compound": "KH2PO4",
            "compound_id": 11,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 0.3,
            "unit": "g",
            "g_l": 0.332594,
            "mmol_l": 2.444,
            "optional": "no"
        },
        {
            "recipe No.": 2,
            "compound": "K2HPO4",
            "compound_id": 10,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 0.3,
            "unit": "g",
            "g_l": 0.332594,
            "mmol_l": 1.90953,
            "optional": "no"
        },
        {
            "recipe No.": 3,
            "compound": "NH4Cl",
            "compound_id": 42,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 1,
            "unit": "g",
            "g_l": 1.10865,
            "mmol_l": 20.7263,
            "optional": "no"
        },
        {
            "recipe No.": 4,
            "compound": "Na2SO4",
            "compound_id": 110,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 3,
            "unit": "g",
            "g_l": 3.32594,
            "mmol_l": 23.4155,
            "optional": "no"
        },
        {
            "recipe No.": 5,
            "compound": "NaCl",
            "compound_id": 43,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 23,
            "unit": "g",
            "g_l": 25.4989,
            "mmol_l": 436.326,
            "optional": "no"
        },
        {
            "recipe No.": 6,
            "compound": "KCl",
            "compound_id": 61,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 0.1,
            "unit": "g",
            "g_l": 0.110865,
            "mmol_l": 1.48712,
            "optional": "no"
        },
        {
            "recipe No.": 7,
            "compound": "CaCl2",
            "compound_id": 81,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 0.1,
            "unit": "g",
            "g_l": 0.110865,
            "mmol_l": 0.998962,
            "optional": "no"
        },
        {
            "recipe No.": 8,
            "compound": "Yeast extract",
            "compound_id": 16,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": "BD-Difco",
            "amount": 0.1,
            "unit": "g",
            "g_l": 0.110865,
            "mmol_l": null,
            "optional": "no"
        },
        {
            "recipe No.": 9,
            "compound": null,
            "compound_id": null,
            "solution": "Trace element solution",
            "solution_id": 4186,
            "condition": null,
            "attribute": null,
            "amount": 1,
            "unit": "ml",
            "g_l": null,
            "mmol_l": null,
            "optional": "no"
        },
        {
            "recipe No.": 10,
            "compound": "L-Cysteine HCl x H2O",
            "compound_id": 770,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 0.5,
            "unit": "g",
            "g_l": 0.554324,
            "mmol_l": null,
            "optional": "no"
        },
        {
            "recipe No.": 11,
            "compound": "Resazurin",
            "compound_id": 25,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 1,
            "unit": "mg",
            "g_l": 0.00110865,
            "mmol_l": 0.00483724,
            "optional": "no"
        },
        {
            "recipe No.": 12,
            "compound": "Distilled water",
            "compound_id": 4,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 900,
            "unit": "ml",
            "g_l": null,
            "mmol_l": null,
            "optional": "no"
        },
        {
            "recipe No.": 13,
            "compound": "Sodium lactate",
            "compound_id": 1392,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": "1 M",
            "amount": 0.1,
            "unit": "ml",
            "g_l": null,
            "mmol_l": null,
            "optional": "no"
        },
        {
            "recipe No.": 14,
            "compound": "MgCl2 x 6 H2O",
            "compound_id": 79,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": "15%",
            "amount": 0.1,
            "unit": "ml",
            "g_l": null,
            "mmol_l": null,
            "optional": "no"
        },
        {
            "recipe No.": 15,
            "compound": "NaHCO3",
            "compound_id": 55,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": "5%",
            "amount": 0.2,
            "unit": "ml",
            "g_l": null,
            "mmol_l": null,
            "optional": "no"
        },
        {
            "recipe No.": 16,
            "compound": "Na2S x 9 H2O",
            "compound_id": 54,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": "2%",
            "amount": 0.1,
            "unit": "ml",
            "g_l": null,
            "mmol_l": null,
            "optional": "no"
        }
    ],
    "steps": [
        {
            "recipe No.": null,
            "step": "Mix ingredients and adjust pH to 7.2. Distribute 0.9 volume of the medium into tubes (e.g., 4.5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave at 110C for 45 min."
        },
        {
            "recipe No.": 13,
            "step": "To complete the medium, add the following stock solutions [Filter-sterilize NaHCO3 solution and store under a N2-CO2 (4:1, v/v) gas mixture. The other solutions are autoclaved and stored anaerobically under a N2 atmosphere] to the medium (per 4.5 ml): "
        }
    ],
    "equipment": {
        "1101": "Autoclave",
        "1357": "Anaerobic workbench",
        "1901": "Hungate tubes",
        "2940": "Filter"
    },
    "Sol. No.": null,
    "main solution": "no"
}