{
    "id": 3266,
    "name": "Main sol. 1579",
    "volume": 1003,
    "recipe": [
        {
            "recipe No.": 1,
            "compound": "NH4Cl",
            "compound_id": 42,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 0.33,
            "unit": "g",
            "g_l": 0.329013,
            "mmol_l": 6.15092,
            "optional": "no"
        },
        {
            "recipe No.": 2,
            "compound": "KCl",
            "compound_id": 61,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 0.33,
            "unit": "g",
            "g_l": 0.329013,
            "mmol_l": 4.41332,
            "optional": "no"
        },
        {
            "recipe No.": 3,
            "compound": "KH2PO4",
            "compound_id": 11,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 0.33,
            "unit": "g",
            "g_l": 0.329013,
            "mmol_l": 2.4177,
            "optional": "no"
        },
        {
            "recipe No.": 4,
            "compound": "MgCl2 x 6 H2O",
            "compound_id": 79,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 0.33,
            "unit": "g",
            "g_l": 0.329013,
            "mmol_l": 1.61834,
            "optional": "no"
        },
        {
            "recipe No.": 5,
            "compound": "CaCl2 x 2 H2O",
            "compound_id": 7,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 0.33,
            "unit": "g",
            "g_l": 0.329013,
            "mmol_l": 2.23796,
            "optional": "no"
        },
        {
            "recipe No.": 6,
            "compound": null,
            "compound_id": null,
            "solution": "Trace element solution SL-10",
            "solution_id": 595,
            "condition": null,
            "attribute": null,
            "amount": 1,
            "unit": "ml",
            "g_l": null,
            "mmol_l": null,
            "optional": "no"
        },
        {
            "recipe No.": 7,
            "compound": null,
            "compound_id": null,
            "solution": "Selenite-tungstate solution",
            "solution_id": 777,
            "condition": null,
            "attribute": null,
            "amount": 1,
            "unit": "ml",
            "g_l": null,
            "mmol_l": null,
            "optional": "no"
        },
        {
            "recipe No.": 8,
            "compound": "Yeast extract",
            "compound_id": 16,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 1,
            "unit": "g",
            "g_l": 0.997009,
            "mmol_l": null,
            "optional": "no"
        },
        {
            "recipe No.": 9,
            "compound": "KNO3",
            "compound_id": 191,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 1,
            "unit": "g",
            "g_l": 0.997009,
            "mmol_l": 9.86132,
            "optional": "no"
        },
        {
            "recipe No.": 10,
            "compound": "NaHCO3",
            "compound_id": 55,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 2,
            "unit": "g",
            "g_l": 1.99402,
            "mmol_l": 23.7363,
            "optional": "no"
        },
        {
            "recipe No.": 11,
            "compound": null,
            "compound_id": null,
            "solution": "Wolin's vitamin solution (10x)",
            "solution_id": 5980,
            "condition": null,
            "attribute": null,
            "amount": 1,
            "unit": "ml",
            "g_l": null,
            "mmol_l": null,
            "optional": "no"
        },
        {
            "recipe No.": 12,
            "compound": "Distilled water",
            "compound_id": 4,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 1000,
            "unit": "ml",
            "g_l": null,
            "mmol_l": null,
            "optional": "no"
        }
    ],
    "steps": [
        {
            "recipe No.": null,
            "step": "Dissolve ingredients (except yeast extract, nitrate, bicarbonate and vitamins) and sparge medium with 100% CO2 gas for at least 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add yeast extract, nitrate and vitamins from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins should be sterilized by filtration. The pH of the complete medium should be adjusted to 6.5 - 6.8, if necessary."
        }
    ],
    "equipment": {
        "925": "Filter",
        "2956": "Autoclave",
        "3746": "Hungate tubes",
        "4204": "Gassing station"
    },
    "Sol. No.": null,
    "main solution": "no"
}