{
    "id": 3150,
    "name": "Main sol. 1523",
    "volume": 1002,
    "recipe": [
        {
            "recipe No.": 1,
            "compound": "KH2PO4",
            "compound_id": 11,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 0.5,
            "unit": "g",
            "g_l": 0.499002,
            "mmol_l": 3.66684,
            "optional": "no"
        },
        {
            "recipe No.": 2,
            "compound": "MgSO4 x 7 H2O",
            "compound_id": 8,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 0.4,
            "unit": "g",
            "g_l": 0.399202,
            "mmol_l": 1.61964,
            "optional": "no"
        },
        {
            "recipe No.": 3,
            "compound": "NaCl",
            "compound_id": 43,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 0.4,
            "unit": "g",
            "g_l": 0.399202,
            "mmol_l": 6.83097,
            "optional": "no"
        },
        {
            "recipe No.": 4,
            "compound": "NH4Cl",
            "compound_id": 42,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 0.4,
            "unit": "g",
            "g_l": 0.399202,
            "mmol_l": 7.46311,
            "optional": "no"
        },
        {
            "recipe No.": 5,
            "compound": "CaCl2 x 2 H2O",
            "compound_id": 7,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 0.05,
            "unit": "g",
            "g_l": 0.0499002,
            "mmol_l": 0.339422,
            "optional": "no"
        },
        {
            "recipe No.": 6,
            "compound": null,
            "compound_id": null,
            "solution": "Trace element solution SL-10",
            "solution_id": 595,
            "condition": null,
            "attribute": null,
            "amount": 1,
            "unit": "ml",
            "g_l": null,
            "mmol_l": null,
            "optional": "no"
        },
        {
            "recipe No.": 7,
            "compound": "Brain heart infusion",
            "compound_id": 186,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": "BD Bacto",
            "amount": 6,
            "unit": "g",
            "g_l": 5.98802,
            "mmol_l": null,
            "optional": "no"
        },
        {
            "recipe No.": 8,
            "compound": "Proteose peptone",
            "compound_id": 208,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": "BD Difco",
            "amount": 6,
            "unit": "g",
            "g_l": 5.98802,
            "mmol_l": null,
            "optional": "no"
        },
        {
            "recipe No.": 9,
            "compound": "Yeast extract",
            "compound_id": 16,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": "OXOID",
            "amount": 2,
            "unit": "g",
            "g_l": 1.99601,
            "mmol_l": null,
            "optional": "no"
        },
        {
            "recipe No.": 10,
            "compound": "Na-acetate",
            "compound_id": 23,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 1,
            "unit": "g",
            "g_l": 0.998004,
            "mmol_l": 12.1658,
            "optional": "no"
        },
        {
            "recipe No.": 11,
            "compound": "Na-formate",
            "compound_id": 144,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 2,
            "unit": "g",
            "g_l": 1.99601,
            "mmol_l": 29.35,
            "optional": "no"
        },
        {
            "recipe No.": 12,
            "compound": "Sodium resazurin",
            "compound_id": 1852,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": "0.1% w/v",
            "amount": 0.5,
            "unit": "ml",
            "g_l": 0.000499002,
            "mmol_l": 0.00198671,
            "optional": "no"
        },
        {
            "recipe No.": 13,
            "compound": "NaHCO3",
            "compound_id": 55,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 4,
            "unit": "g",
            "g_l": 3.99202,
            "mmol_l": 47.52,
            "optional": "no"
        },
        {
            "recipe No.": 14,
            "compound": null,
            "compound_id": null,
            "solution": "Seven vitamins solution",
            "solution_id": 1038,
            "condition": null,
            "attribute": null,
            "amount": 1,
            "unit": "ml",
            "g_l": null,
            "mmol_l": null,
            "optional": "no"
        },
        {
            "recipe No.": 15,
            "compound": "L-Cysteine HCl x H2O",
            "compound_id": 770,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 0.5,
            "unit": "g",
            "g_l": 0.499002,
            "mmol_l": 2.84111,
            "optional": "no"
        },
        {
            "recipe No.": 16,
            "compound": "Na2S x 9 H2O",
            "compound_id": 54,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 0.5,
            "unit": "g",
            "g_l": 0.499002,
            "mmol_l": 2.0776,
            "optional": "no"
        },
        {
            "recipe No.": 17,
            "compound": "Distilled water",
            "compound_id": 4,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 1000,
            "unit": "ml",
            "g_l": null,
            "mmol_l": null,
            "optional": "no"
        }
    ],
    "steps": [
        {
            "recipe No.": null,
            "step": "Dissolve ingredients except bicarbonate, vitamins, cysteine, and sulfide, then sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve bicarbonate and adjust pH to 6.5. Then dispense medium under 80% H2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Add vitamins, cysteine, and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The vitamin solution should be sterilized by filtration. Before use, check the complete medium's pH and adjust it to 6.8 - 7.0, if necessary."
        },
        {
            "recipe No.": null,
            "step": "After growth has started and the culture is becoming turbid add sterile 80% H2 and 20% CO2 gas mixture to 0.5 - 1 bar overpressure."
        }
    ],
    "equipment": {
        "155": "Hungate tubes",
        "1226": "Filter",
        "3215": "Autoclave",
        "4189": "Gassing station"
    },
    "Sol. No.": null,
    "main solution": "no"
}