{
    "id": 1875,
    "name": "Main sol. 913",
    "volume": 1001,
    "recipe": [
        {
            "recipe No.": 1,
            "compound": null,
            "compound_id": null,
            "solution": "Artificial sea water",
            "solution_id": 659,
            "condition": null,
            "attribute": null,
            "amount": 750,
            "unit": "ml",
            "g_l": null,
            "mmol_l": null,
            "optional": "no"
        },
        {
            "recipe No.": 2,
            "compound": "(NH4)2SO4",
            "compound_id": 27,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 1,
            "unit": "g",
            "g_l": 0.999001,
            "mmol_l": 7.56017,
            "optional": "no"
        },
        {
            "recipe No.": 3,
            "compound": "KH2PO4",
            "compound_id": 11,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 0.5,
            "unit": "g",
            "g_l": 0.499501,
            "mmol_l": 3.6705,
            "optional": "no"
        },
        {
            "recipe No.": 4,
            "compound": "Yeast extract",
            "compound_id": 16,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 2,
            "unit": "g",
            "g_l": 1.998,
            "mmol_l": null,
            "optional": "no"
        },
        {
            "recipe No.": 5,
            "compound": "MOPS",
            "compound_id": 351,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": "SIGMA",
            "amount": 5,
            "unit": "g",
            "g_l": 4.99501,
            "mmol_l": 23.8699,
            "optional": "no"
        },
        {
            "recipe No.": 6,
            "compound": null,
            "compound_id": null,
            "solution": "Modified Wolin's mineral solution",
            "solution_id": 241,
            "condition": null,
            "attribute": null,
            "amount": 10,
            "unit": "ml",
            "g_l": null,
            "mmol_l": null,
            "optional": "no"
        },
        {
            "recipe No.": 7,
            "compound": "Sodium resazurin",
            "compound_id": 1852,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": "0.1% w/v",
            "amount": 0.5,
            "unit": "ml",
            "g_l": 0.0004995,
            "mmol_l": 0.0019887,
            "optional": "no"
        },
        {
            "recipe No.": 8,
            "compound": null,
            "compound_id": null,
            "solution": "Wolin's vitamin solution (10x)",
            "solution_id": 5980,
            "condition": null,
            "attribute": null,
            "amount": 1,
            "unit": "ml",
            "g_l": null,
            "mmol_l": null,
            "optional": "no"
        },
        {
            "recipe No.": 9,
            "compound": "Na2S x 9 H2O",
            "compound_id": 54,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 0.3,
            "unit": "g",
            "g_l": 0.2997,
            "mmol_l": 1.2478,
            "optional": "no"
        },
        {
            "recipe No.": 10,
            "compound": "Distilled water",
            "compound_id": 4,
            "solution": null,
            "solution_id": null,
            "condition": null,
            "attribute": null,
            "amount": 240,
            "unit": "ml",
            "g_l": null,
            "mmol_l": null,
            "optional": "no"
        }
    ],
    "steps": [
        {
            "recipe No.": null,
            "step": "Dissolve ingredients except vitamins and sulfide, adjust pH to 7.0, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials (to 30% of volume) and autoclave. Add vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins should be sterilized by filtration. Adjust pH of the complete medium to 7.0, if necessary."
        }
    ],
    "equipment": {
        "930": "Filter",
        "2959": "Autoclave",
        "3751": "Hungate tubes",
        "4482": "Gassing station"
    },
    "Sol. No.": null,
    "main solution": "no"
}