{"medium":{"id":"J677","name":"BRUCELLA BLOOD AGAR WITH HEMIN MENADIONE","complex_medium":"yes","source":"JCM","link":"https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=677"},"solutions":[{"id":4574,"name":"Main sol. J677","volume":1070,"recipe":[{"recipe No.":1,"compound":"Brucella agar","compound_id":1854,"attribute":"BD-BBL","amount":43,"unit":"g","g_l":40.1869,"optional":"no"},{"recipe No.":2,"compound":"Horse blood","compound_id":724,"amount":50,"unit":"ml","optional":"no"},{"recipe No.":3,"solution":"Hemin solution","solution_id":4218,"amount":10,"unit":"ml","optional":"no"},{"recipe No.":4,"solution":"Hemin solution","solution_id":4218,"amount":10,"unit":"ml","optional":"no"},{"recipe No.":5,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"main solution":"yes"},{"id":4218,"name":"Hemin solution","steps":[{"step":"Dissolve 50 mg hemin first in 1 ml of 1 N NaOH. Then adjust volume to 100 ml."}],"main solution":"no"}]}