{"medium":{"id":"J14","name":"EG MEDIUM","complex_medium":"yes","min_pH":7.7,"max_pH":7.7,"source":"JCM","link":"https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=14"},"solutions":[{"id":3635,"name":"Main sol. J14","volume":1000,"recipe":[{"recipe No.":1,"compound":"Lab-Lemco beef extract","compound_id":599,"attribute":"Oxoid","amount":2.4,"unit":"g","g_l":2.4,"optional":"no"},{"recipe No.":2,"compound":"Proteose peptone no. 3","compound_id":316,"attribute":"BD-Difco","amount":10,"unit":"g","g_l":10,"optional":"no"},{"recipe No.":3,"compound":"Yeast extract","compound_id":16,"attribute":"BD-Difco","amount":5,"unit":"g","g_l":5,"optional":"no"},{"recipe No.":4,"compound":"Na2HPO4","compound_id":233,"amount":4,"unit":"g","g_l":4,"mmol_l":28.1771,"optional":"no"},{"recipe No.":5,"compound":"Glucose","compound_id":5,"amount":1.5,"unit":"g","g_l":1.5,"mmol_l":8.32593,"optional":"no"},{"recipe No.":6,"compound":"Starch","compound_id":199,"attribute":"soluble","amount":0.5,"unit":"g","g_l":0.5,"optional":"no"},{"recipe No.":7,"compound":"L-Cystine","compound_id":267,"amount":0.2,"unit":"g","g_l":0.2,"mmol_l":0.832293,"optional":"no"},{"recipe No.":8,"compound":"Agar","compound_id":3,"amount":15,"unit":"g","g_l":15,"mmol_l":44.5991,"optional":"no"},{"recipe No.":9,"compound":"L-Cysteine HCl x H2O","compound_id":770,"amount":0.5,"unit":"g","g_l":0.5,"optional":"no"},{"recipe No.":10,"compound":"Horse blood","compound_id":724,"amount":50,"unit":"ml","optional":"no"},{"recipe No.":11,"compound":"Distilled water","compound_id":4,"amount":950,"unit":"ml","optional":"no"}],"steps":[{"step":"Add L-cystine to 50 ml of 1 N HCl and mix thoroughly. Add the remaining components and bring volume to 950 ml. Adjust pH to 7.6-7.8. After autoclaving and then cooling to 50C, aseptically add 50.0 ml of horse blood. Mix thoroughly and pour into sterile petri dishes or distribute into sterile tubes."}],"main solution":"yes"}]}