{"medium":{"id":"J1282","name":"HNN MEDIUM","complex_medium":"no","source":"JCM","link":"https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1282"},"solutions":[{"id":5426,"name":"Main sol. J1282","volume":1010,"recipe":[{"recipe No.":1,"compound":"NaCl","compound_id":43,"amount":25,"unit":"g","g_l":24.7525,"mmol_l":423.554,"optional":"no"},{"recipe No.":2,"compound":"MgCl2 x 6 H2O","compound_id":79,"amount":4.2,"unit":"g","g_l":4.15842,"mmol_l":20.4543,"optional":"no"},{"recipe No.":3,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":3.4,"unit":"g","g_l":3.36634,"mmol_l":13.6579,"optional":"no"},{"recipe No.":4,"compound":"KCl","compound_id":61,"amount":0.5,"unit":"g","g_l":0.49505,"mmol_l":6.6405,"optional":"no"},{"recipe No.":5,"compound":"NH4Cl","compound_id":42,"amount":0.25,"unit":"g","g_l":0.247525,"mmol_l":4.6275,"optional":"no"},{"recipe No.":6,"compound":"K2HPO4","compound_id":10,"amount":0.14,"unit":"g","g_l":0.138614,"mmol_l":0.795826,"optional":"no"},{"recipe No.":7,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":0.7,"unit":"g","g_l":0.693069,"mmol_l":4.71428,"optional":"no"},{"recipe No.":8,"compound":"NaHCO3","compound_id":55,"amount":1,"unit":"g","g_l":0.990099,"mmol_l":11.7859,"optional":"no"},{"recipe No.":9,"solution":"Trace mineral solution","solution_id":5427,"amount":10,"unit":"ml","optional":"no"},{"recipe No.":10,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Mix components, except NaHCO3, and autoclave under N2 atmosphere. Filter-sterilize 8% (w/v) NaHCO3 solution and add to the medium. Aseptically distribute the medium into culture vessels and seal with butyl rubber stoppers. Replace gas phase with N2O, After inoculation, pressurize the inoculated bottles to 300 kPa H2-CO2 (4:1, v/v)."}],"equipment":{"800":"Autoclave","2664":"Filter"},"main solution":"yes"},{"id":5427,"name":"Trace mineral solution","volume":1000,"recipe":[{"recipe No.":1,"solution":"Trace minerals","solution_id":3804,"amount":1000,"unit":"ml","optional":"no"},{"recipe No.":2,"compound":"NiCl2 x 6 H2O","compound_id":40,"amount":0.025,"unit":"g","g_l":0.025,"mmol_l":0.192903,"optional":"no"},{"recipe No.":3,"compound":"Na2SeO3 x 5 H2O","compound_id":107,"amount":0.3,"unit":"mg","g_l":0.0003,"mmol_l":0.00114062,"optional":"no"},{"recipe No.":4,"compound":"Na2WO4 x 2 H2O","compound_id":249,"amount":0.1,"unit":"g","g_l":0.1,"mmol_l":0.303168,"optional":"no"}],"main solution":"no"},{"id":3804,"name":"Trace minerals","volume":1000,"recipe":[{"recipe No.":1,"compound":"Nitrilotriacetic acid","compound_id":189,"amount":1.5,"unit":"g","g_l":1.5,"mmol_l":7.84765,"optional":"no"},{"recipe No.":2,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":3,"unit":"g","g_l":3,"mmol_l":12.1716,"optional":"no"},{"recipe No.":3,"compound":"MnSO4 x n H2O","compound_id":1000,"amount":0.5,"unit":"g","g_l":0.5,"optional":"no"},{"recipe No.":4,"compound":"NaCl","compound_id":43,"amount":1,"unit":"g","g_l":1,"mmol_l":17.1116,"optional":"no"},{"recipe No.":5,"compound":"FeSO4 x 7 H2O","compound_id":12,"amount":0.1,"unit":"g","g_l":0.1,"mmol_l":0.359693,"optional":"no"},{"recipe No.":6,"compound":"CoSO4 x 7 H2O","compound_id":198,"amount":0.1,"unit":"g","g_l":0.1,"mmol_l":0.645178,"optional":"no"},{"recipe No.":7,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":0.1,"unit":"g","g_l":0.1,"mmol_l":0.680203,"optional":"no"},{"recipe No.":8,"compound":"ZnSO4 x 7 H2O","compound_id":35,"amount":0.1,"unit":"g","g_l":0.1,"mmol_l":0.347752,"optional":"no"},{"recipe No.":9,"compound":"CuSO4 x 5 H2O","compound_id":121,"amount":0.01,"unit":"g","g_l":0.01,"mmol_l":0.0400503,"optional":"no"},{"recipe No.":10,"compound":"AlK(SO4)2","compound_id":1015,"amount":0.01,"unit":"g","g_l":0.01,"mmol_l":0.0387289,"optional":"no"},{"recipe No.":11,"compound":"H3BO3","compound_id":37,"amount":0.01,"unit":"g","g_l":0.01,"mmol_l":0.161708,"optional":"no"},{"recipe No.":12,"compound":"Na2MoO4 x 2 H2O","compound_id":9,"amount":0.01,"unit":"g","g_l":0.01,"mmol_l":0.0413309,"optional":"no"},{"recipe No.":13,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0."}],"main solution":"no"}]}