{"medium":{"id":95,"name":"DESULFUROMONAS ACETOXIDANS MEDIUM","complex_medium":"yes","min_pH":7.2,"max_pH":7.2,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium95.pdf"},"solutions":[{"id":164,"name":"Main sol. 95","volume":1004,"recipe":[{"recipe No.":1,"solution":"Solution A","solution_id":165,"amount":951,"unit":"ml","optional":"no"},{"recipe No.":2,"solution":"Solution B","solution_id":166,"amount":3,"unit":"ml","optional":"no"},{"recipe No.":3,"solution":"Solution C","solution_id":167,"amount":40,"unit":"ml","optional":"no"},{"recipe No.":4,"solution":"Solution D","solution_id":168,"amount":10,"unit":"ml","optional":"no"}],"steps":[{"step":"Sparge solution A with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense solution under same gas atmosphere into anoxic Hungate-type tubes or serum vials and sterilize by autoclaving. Solutions B and D are autoclaved separately under 100% N2 gas atmosphere. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. The medium is completed before use by adding appropriate amounts of solutions B, C and D to solution A. Adjust pH of complete medium to 7.2, if necessary."}],"equipment":{"3971":"Autoclave","3972":"Gassing station","3973":"Hungate tubes"},"main solution":"yes"},{"id":165,"name":"Solution A","volume":951,"recipe":[{"recipe No.":1,"compound":"KH2PO4","compound_id":11,"amount":1,"unit":"g","g_l":1.05152,"mmol_l":7.72697,"optional":"no"},{"recipe No.":2,"compound":"NH4Cl","compound_id":42,"amount":0.3,"unit":"g","g_l":0.315457,"mmol_l":5.8975,"optional":"no"},{"recipe No.":3,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":1,"unit":"g","g_l":1.05152,"mmol_l":4.26624,"optional":"no"},{"recipe No.":4,"compound":"MgCl2 x 6 H2O","compound_id":79,"amount":2,"unit":"g","g_l":2.10305,"mmol_l":10.3444,"optional":"no"},{"recipe No.":5,"compound":"NaCl","compound_id":43,"amount":20,"unit":"g","g_l":21.0305,"mmol_l":359.865,"optional":"no"},{"recipe No.":6,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":0.1,"unit":"g","g_l":0.105152,"mmol_l":0.71525,"optional":"no"},{"recipe No.":7,"compound":"Na2SO4","compound_id":110,"amount":1.77,"unit":"g","g_l":1.8612,"mmol_l":13.1033,"optional":"no"},{"recipe No.":8,"solution":"Trace element solution SL-11","solution_id":1474,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":9,"compound":"Na2-DL-malate","compound_id":204,"amount":2.66,"unit":"g","g_l":2.79706,"mmol_l":15.7093,"optional":"no"},{"recipe No.":10,"compound":"Yeast extract","compound_id":16,"amount":0.5,"unit":"g","g_l":0.525762,"optional":"no"},{"recipe No.":11,"compound":"Sodium resazurin","compound_id":1852,"attribute":"0.1% w/v","amount":0.5,"unit":"ml","g_l":0.000525762,"mmol_l":0.00209325,"optional":"no"},{"recipe No.":12,"compound":"Distilled water","compound_id":4,"amount":950,"unit":"ml","optional":"no"}],"steps":[{"step":"Adjust pH to 6.0 with 2 N NaOH."}],"main solution":"no"},{"id":166,"name":"Solution B","volume":3,"recipe":[{"recipe No.":1,"compound":"Ethanol","compound_id":201,"amount":0.3,"unit":"ml","optional":"no"},{"recipe No.":2,"compound":"Distilled water","compound_id":4,"amount":2.7,"unit":"ml","optional":"no"}],"main solution":"no"},{"id":167,"name":"Solution C","volume":40,"recipe":[{"recipe No.":1,"compound":"NaHCO3","compound_id":55,"amount":1.85,"unit":"g","g_l":46.25,"mmol_l":550.549,"optional":"no"},{"recipe No.":2,"compound":"Distilled water","compound_id":4,"amount":40,"unit":"ml","optional":"no"}],"main solution":"no"},{"id":168,"name":"Solution D","volume":10,"recipe":[{"recipe No.":1,"compound":"Na2S x 9 H2O","compound_id":54,"amount":0.3,"unit":"g","g_l":30,"mmol_l":124.905,"optional":"no"},{"recipe No.":2,"compound":"Distilled water","compound_id":4,"amount":10,"unit":"ml","optional":"no"}],"equipment":{"1129":"Autoclave","1924":"Hungate tubes"},"main solution":"no"},{"id":1474,"name":"Trace element solution SL-11","volume":1000,"recipe":[{"recipe No.":1,"compound":"Na2-EDTA x 2 H2O","compound_id":676,"amount":5.2,"unit":"g","g_l":5.2,"mmol_l":15.4667,"optional":"no"},{"recipe No.":2,"compound":"FeCl2 x 4 H2O","compound_id":260,"amount":1.5,"unit":"g","g_l":1.5,"mmol_l":7.54482,"optional":"no"},{"recipe No.":3,"compound":"ZnCl2","compound_id":64,"amount":70,"unit":"mg","g_l":0.07,"mmol_l":0.513573,"optional":"no"},{"recipe No.":4,"compound":"MnCl2 x 4 H2O","compound_id":36,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.505293,"optional":"no"},{"recipe No.":5,"compound":"H3BO3","compound_id":37,"amount":6,"unit":"mg","g_l":0.006,"mmol_l":0.0970246,"optional":"no"},{"recipe No.":6,"compound":"CoCl2 x 6 H2O","compound_id":38,"amount":190,"unit":"mg","g_l":0.19,"mmol_l":1.46335,"optional":"no"},{"recipe No.":7,"compound":"CuCl2 x 2 H2O","compound_id":39,"amount":2,"unit":"mg","g_l":0.002,"mmol_l":0.0117314,"optional":"no"},{"recipe No.":8,"compound":"NiCl2 x 6 H2O","compound_id":40,"amount":24,"unit":"mg","g_l":0.024,"mmol_l":0.185187,"optional":"no"},{"recipe No.":9,"compound":"Na2MoO4 x 2 H2O","compound_id":9,"amount":36,"unit":"mg","g_l":0.036,"mmol_l":0.148791,"optional":"no"},{"recipe No.":10,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml. "}],"Sol. No.":13,"main solution":"no"}]}