{"medium":{"id":913,"name":"THERMOTOGA PETROPHILA MEDIUM","complex_medium":"yes","min_pH":7,"max_pH":7,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium913.pdf"},"solutions":[{"id":1875,"name":"Main sol. 913","volume":1001,"recipe":[{"recipe No.":1,"solution":"Artificial sea water","solution_id":659,"amount":750,"unit":"ml","optional":"no"},{"recipe No.":2,"compound":"(NH4)2SO4","compound_id":27,"amount":1,"unit":"g","g_l":0.999001,"mmol_l":7.56017,"optional":"no"},{"recipe No.":3,"compound":"KH2PO4","compound_id":11,"amount":0.5,"unit":"g","g_l":0.499501,"mmol_l":3.6705,"optional":"no"},{"recipe No.":4,"compound":"Yeast extract","compound_id":16,"amount":2,"unit":"g","g_l":1.998,"optional":"no"},{"recipe No.":5,"compound":"MOPS","compound_id":351,"attribute":"SIGMA","amount":5,"unit":"g","g_l":4.99501,"mmol_l":23.8699,"optional":"no"},{"recipe No.":6,"solution":"Modified Wolin's mineral solution","solution_id":241,"amount":10,"unit":"ml","optional":"no"},{"recipe No.":7,"compound":"Sodium resazurin","compound_id":1852,"attribute":"0.1% w/v","amount":0.5,"unit":"ml","g_l":0.0004995,"mmol_l":0.0019887,"optional":"no"},{"recipe No.":8,"solution":"Wolin's vitamin solution (10x)","solution_id":5980,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":9,"compound":"Na2S x 9 H2O","compound_id":54,"amount":0.3,"unit":"g","g_l":0.2997,"mmol_l":1.2478,"optional":"no"},{"recipe No.":10,"compound":"Distilled water","compound_id":4,"amount":240,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve ingredients except vitamins and sulfide, adjust pH to 7.0, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials (to 30% of volume) and autoclave. Add vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins should be sterilized by filtration. Adjust pH of the complete medium to 7.0, if necessary."}],"equipment":{"930":"Filter","2959":"Autoclave","3751":"Hungate tubes","4482":"Gassing station"},"main solution":"yes"},{"id":659,"name":"Artificial sea water","volume":1000,"recipe":[{"recipe No.":1,"compound":"NaCl","compound_id":43,"amount":27.7,"unit":"g","g_l":27.7,"mmol_l":473.99,"optional":"no"},{"recipe No.":2,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":7,"unit":"g","g_l":7,"mmol_l":28.4003,"optional":"no"},{"recipe No.":3,"compound":"MgCl2 x 6 H2O","compound_id":79,"amount":5.5,"unit":"g","g_l":5.5,"mmol_l":27.0532,"optional":"no"},{"recipe No.":4,"compound":"KCl","compound_id":61,"amount":0.65,"unit":"g","g_l":0.65,"mmol_l":8.71898,"optional":"no"},{"recipe No.":5,"compound":"NaBr","compound_id":353,"amount":0.1,"unit":"g","g_l":0.1,"mmol_l":0.971912,"optional":"no"},{"recipe No.":6,"compound":"H3BO3","compound_id":37,"amount":30,"unit":"mg","g_l":0.03,"mmol_l":0.485123,"optional":"no"},{"recipe No.":7,"compound":"SrCl2 x 6 H2O","compound_id":176,"amount":15,"unit":"mg","g_l":0.015,"mmol_l":0.0562605,"optional":"no"},{"recipe No.":8,"compound":"Citric acid","compound_id":348,"amount":10,"unit":"mg","g_l":0.01,"mmol_l":0.0520508,"optional":"no"},{"recipe No.":9,"compound":"KI","compound_id":354,"amount":0.05,"unit":"mg","g_l":5.0e-5,"mmol_l":0.000192793,"optional":"no"},{"recipe No.":10,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":2.25,"unit":"g","g_l":2.25,"mmol_l":15.3046,"optional":"no"},{"recipe No.":11,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"Sol. No.":27,"main solution":"no"},{"id":241,"name":"Modified Wolin's mineral solution","volume":1000,"recipe":[{"recipe No.":1,"compound":"Nitrilotriacetic acid","compound_id":189,"amount":1.5,"unit":"g","g_l":1.5,"mmol_l":7.84765,"optional":"no"},{"recipe No.":2,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":3,"unit":"g","g_l":3,"mmol_l":12.1716,"optional":"no"},{"recipe No.":3,"compound":"MnSO4 x H2O","compound_id":14,"amount":0.5,"unit":"g","g_l":0.5,"mmol_l":2.9583,"optional":"no"},{"recipe No.":4,"compound":"NaCl","compound_id":43,"amount":1,"unit":"g","g_l":1,"mmol_l":17.1116,"optional":"no"},{"recipe No.":5,"compound":"FeSO4 x 7 H2O","compound_id":12,"amount":0.1,"unit":"g","g_l":0.1,"mmol_l":0.359693,"optional":"no"},{"recipe No.":6,"compound":"CoSO4 x 7 H2O","compound_id":198,"amount":0.18,"unit":"g","g_l":0.18,"mmol_l":1.16132,"optional":"no"},{"recipe No.":7,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":0.1,"unit":"g","g_l":0.1,"mmol_l":0.680203,"optional":"no"},{"recipe No.":8,"compound":"ZnSO4 x 7 H2O","compound_id":35,"amount":0.18,"unit":"g","g_l":0.18,"mmol_l":0.625954,"optional":"no"},{"recipe No.":9,"compound":"CuSO4 x 5 H2O","compound_id":121,"amount":0.01,"unit":"g","g_l":0.01,"mmol_l":0.0400503,"optional":"no"},{"recipe No.":10,"compound":"AlK(SO4)2 x 12 H2O","compound_id":908,"amount":0.02,"unit":"g","g_l":0.02,"mmol_l":0.0421596,"optional":"no"},{"recipe No.":11,"compound":"H3BO3","compound_id":37,"amount":0.01,"unit":"g","g_l":0.01,"mmol_l":0.161708,"optional":"no"},{"recipe No.":12,"compound":"Na2MoO4 x 2 H2O","compound_id":9,"amount":0.01,"unit":"g","g_l":0.01,"mmol_l":0.0413309,"optional":"no"},{"recipe No.":13,"compound":"NiCl2 x 6 H2O","compound_id":40,"amount":0.03,"unit":"g","g_l":0.03,"mmol_l":0.231483,"optional":"no"},{"recipe No.":14,"compound":"Na2SeO3 x 5 H2O","compound_id":107,"amount":0.3,"unit":"mg","g_l":0.0003,"mmol_l":0.00114062,"optional":"no"},{"recipe No.":15,"compound":"Na2WO4 x 2 H2O","compound_id":249,"amount":0.4,"unit":"mg","g_l":0.0004,"mmol_l":0.00121267,"optional":"no"},{"recipe No.":16,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH."}],"Sol. No.":17,"main solution":"no"},{"id":5980,"name":"Wolin's vitamin solution (10x)","volume":1000,"recipe":[{"recipe No.":1,"compound":"Biotin","compound_id":46,"amount":20,"unit":"mg","g_l":0.02,"mmol_l":0.0818632,"optional":"no"},{"recipe No.":2,"compound":"Folic acid","compound_id":138,"amount":20,"unit":"mg","g_l":0.02,"mmol_l":0.0453104,"optional":"no"},{"recipe No.":3,"compound":"Pyridoxine hydrochloride","compound_id":519,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.486287,"optional":"no"},{"recipe No.":4,"compound":"Thiamine HCl","compound_id":1207,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.148236,"optional":"no"},{"recipe No.":5,"compound":"Riboflavin","compound_id":136,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.132837,"optional":"no"},{"recipe No.":6,"compound":"Nicotinic acid","compound_id":139,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.406141,"optional":"no"},{"recipe No.":7,"compound":"Calcium D-(+)-pantothenate","compound_id":1512,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.209846,"optional":"no"},{"recipe No.":8,"compound":"Vitamin B12","compound_id":18,"amount":1,"unit":"mg","g_l":0.001,"mmol_l":0.00073779,"optional":"no"},{"recipe No.":9,"compound":"p-Aminobenzoic acid","compound_id":47,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.364591,"optional":"no"},{"recipe No.":10,"compound":"(DL)-alpha-Lipoic acid","compound_id":252,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.24233,"optional":"no"},{"recipe No.":11,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"main solution":"no"}]}