{"medium":{"id":881,"name":"DENITROVIBRIO MEDIUM","complex_medium":"no","min_pH":7,"max_pH":7.2,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium881.pdf"},"solutions":[{"id":1822,"name":"Main sol. 881","volume":1003,"recipe":[{"recipe No.":1,"compound":"Na2SO4","compound_id":110,"amount":0.02,"unit":"g","g_l":0.0199402,"mmol_l":0.140384,"optional":"no"},{"recipe No.":2,"compound":"KH2PO4","compound_id":11,"amount":1,"unit":"g","g_l":0.997009,"mmol_l":7.32637,"optional":"no"},{"recipe No.":3,"compound":"NH4Cl","compound_id":42,"amount":0.25,"unit":"g","g_l":0.249252,"mmol_l":4.65979,"optional":"no"},{"recipe No.":4,"compound":"NaCl","compound_id":43,"amount":20,"unit":"g","g_l":19.9402,"mmol_l":341.208,"optional":"no"},{"recipe No.":5,"compound":"MgCl2 x 6 H2O","compound_id":79,"amount":3,"unit":"g","g_l":2.99103,"mmol_l":14.7122,"optional":"no"},{"recipe No.":6,"compound":"KCl","compound_id":61,"amount":0.5,"unit":"g","g_l":0.498504,"mmol_l":6.68685,"optional":"no"},{"recipe No.":7,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":0.15,"unit":"g","g_l":0.149551,"mmol_l":1.01725,"optional":"no"},{"recipe No.":8,"compound":"Na-acetate","compound_id":23,"amount":1.64,"unit":"g","g_l":1.63509,"mmol_l":19.932,"optional":"no"},{"recipe No.":9,"solution":"Trace element solution SL-10","solution_id":595,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":10,"solution":"Selenite-tungstate solution","solution_id":777,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":11,"compound":"Sodium resazurin","compound_id":1852,"attribute":"0.1% w/v","amount":0.5,"unit":"ml","g_l":0.000498504,"mmol_l":0.00198473,"optional":"no"},{"recipe No.":12,"compound":"Na2CO3","compound_id":32,"amount":1,"unit":"g","g_l":0.997009,"mmol_l":9.40681,"optional":"no"},{"recipe No.":13,"compound":"Na2-fumarate","compound_id":276,"amount":6.4,"unit":"g","g_l":6.38086,"mmol_l":39.8714,"optional":"no"},{"recipe No.":14,"solution":"Seven vitamins solution","solution_id":1038,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":15,"compound":"Na2S x 9 H2O","compound_id":54,"amount":0.5,"unit":"g","g_l":0.498504,"mmol_l":2.07553,"optional":"no"},{"recipe No.":16,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve all ingredients except carbonate, fumarate, vitamins and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add fumarate, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins should be sterilized by filtration. Adjust pH of complete medium to 7.0 - 7.2, if necessary."}],"equipment":{"1507":"Autoclave","2302":"Hungate tubes","3328":"Filter","4462":"Gassing station"},"main solution":"yes"},{"id":595,"name":"Trace element solution SL-10","volume":1000,"recipe":[{"recipe No.":1,"compound":"HCl","compound_id":68,"attribute":"25%","amount":10,"unit":"ml","g_l":2.5,"mmol_l":68.5683,"optional":"no"},{"recipe No.":2,"compound":"FeCl2 x 4 H2O","compound_id":260,"amount":1.5,"unit":"g","g_l":1.5,"mmol_l":7.54482,"optional":"no"},{"recipe No.":3,"compound":"ZnCl2","compound_id":64,"amount":70,"unit":"mg","g_l":0.07,"mmol_l":0.513573,"optional":"no"},{"recipe No.":4,"compound":"MnCl2 x 4 H2O","compound_id":36,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.505293,"optional":"no"},{"recipe No.":5,"compound":"H3BO3","compound_id":37,"amount":6,"unit":"mg","g_l":0.006,"mmol_l":0.0970246,"optional":"no"},{"recipe No.":6,"compound":"CoCl2 x 6 H2O","compound_id":38,"amount":190,"unit":"mg","g_l":0.19,"mmol_l":1.46335,"optional":"no"},{"recipe No.":7,"compound":"CuCl2 x 2 H2O","compound_id":39,"amount":2,"unit":"mg","g_l":0.002,"mmol_l":0.0117314,"optional":"no"},{"recipe No.":8,"compound":"NiCl2 x 6 H2O","compound_id":40,"amount":24,"unit":"mg","g_l":0.024,"mmol_l":0.185187,"optional":"no"},{"recipe No.":9,"compound":"Na2MoO4 x 2 H2O","compound_id":9,"amount":36,"unit":"mg","g_l":0.036,"mmol_l":0.148791,"optional":"no"},{"recipe No.":10,"compound":"Distilled water","compound_id":4,"amount":990,"unit":"ml","optional":"no"}],"steps":[{"step":"First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml."}],"Sol. No.":2,"main solution":"no"},{"id":777,"name":"Selenite-tungstate solution","volume":1000,"recipe":[{"recipe No.":1,"compound":"NaOH","compound_id":256,"amount":0.5,"unit":"g","g_l":0.5,"mmol_l":12.5009,"optional":"no"},{"recipe No.":2,"compound":"Na2SeO3 x 5 H2O","compound_id":107,"amount":3,"unit":"mg","g_l":0.003,"mmol_l":0.0114062,"optional":"no"},{"recipe No.":3,"compound":"Na2WO4 x 2 H2O","compound_id":249,"amount":4,"unit":"mg","g_l":0.004,"mmol_l":0.0121267,"optional":"no"},{"recipe No.":4,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"Sol. No.":3,"main solution":"no"},{"id":1038,"name":"Seven vitamins solution","volume":1000,"recipe":[{"recipe No.":1,"compound":"Vitamin B12","compound_id":18,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.073779,"optional":"no"},{"recipe No.":2,"compound":"p-Aminobenzoic acid","compound_id":47,"amount":80,"unit":"mg","g_l":0.08,"mmol_l":0.583345,"optional":"no"},{"recipe No.":3,"compound":"D-(+)-biotin","compound_id":1359,"amount":20,"unit":"mg","g_l":0.02,"mmol_l":0.0818632,"optional":"no"},{"recipe No.":4,"compound":"Nicotinic acid","compound_id":139,"amount":200,"unit":"mg","g_l":0.2,"mmol_l":1.62456,"optional":"no"},{"recipe No.":5,"compound":"Calcium pantothenate","compound_id":518,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.209849,"optional":"no"},{"recipe No.":6,"compound":"Pyridoxine hydrochloride","compound_id":519,"amount":300,"unit":"mg","g_l":0.3,"mmol_l":1.45886,"optional":"no"},{"recipe No.":7,"compound":"Thiamine-HCl x 2 H2O","compound_id":77,"amount":200,"unit":"mg","g_l":0.2,"mmol_l":0.535761,"optional":"no"},{"recipe No.":8,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"Sol. No.":4,"main solution":"no"}]}