{"medium":{"id":863,"name":"METHANOTHERMOBACTER/METHANOBACTERIUM TRANSDUCTION MEDIUM","complex_medium":"no","min_pH":7,"max_pH":7,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium863.pdf"},"solutions":[{"id":1780,"name":"Main sol. 863","volume":1016,"recipe":[{"recipe No.":1,"solution":"Basal salts solution","solution_id":1781,"amount":100,"unit":"ml","optional":"no"},{"recipe No.":2,"solution":"Trace elements solution","solution_id":1782,"amount":10,"unit":"ml","optional":"no"},{"recipe No.":3,"compound":"Sodium carbonate solution","compound_id":797,"amount":24,"unit":"ml","g_l":59.7638,"optional":"no"},{"recipe No.":4,"solution":"Cysteine solutions","solution_id":1784,"amount":5,"unit":"ml","optional":"no"},{"recipe No.":5,"solution":"Na2S Solution","solution_id":5833,"amount":5,"unit":"ml","optional":"no"},{"recipe No.":6,"compound":"Distilled water","compound_id":4,"attribute":"degassed","amount":856,"unit":"ml","optional":"no"},{"recipe No.":7,"compound":"Agar","compound_id":3,"amount":15,"unit":"g","g_l":14.7638,"mmol_l":43.8967,"optional":"no"},{"recipe No.":8,"solution":"Titanium (III) citrate solution (Zehnder and Wuhrmann 1976)","solution_id":5663,"amount":16,"unit":"ml","optional":"no"}],"steps":[{"recipe No.":7,"step":"Anaerobically dispense liquid minimal medium in 10-ml portions into 100-ml serum vials under an atmosphere of N2/CO2 (80:20). Seal vials with butyl rubber stoppers secured with aluminium crimp seals. Sterilize by autoclaving for 15 minutes at 121\u00b0C. Prior to inoculation, pressurize bottles to 2 x 102 kPa with H2/CO2. For solidified plating medium, add the following components per liter of liquid minimal medium: "},{"step":"Add titanium(III) citrate solution to warm (60\u00b0C) agar medium after autoclaving. For preparation of solidified agar medium."}],"equipment":{"1654":"Serum bottle","4452":"Gassing station","4990":"Autoclave"},"main solution":"yes"},{"id":1781,"name":"Basal salts solution","volume":1000,"recipe":[{"recipe No.":1,"compound":"KH2PO4","compound_id":11,"amount":68,"unit":"g","g_l":68,"mmol_l":499.688,"optional":"no"},{"recipe No.":2,"compound":"NH4Cl","compound_id":42,"amount":21.2,"unit":"g","g_l":21.2,"mmol_l":396.336,"optional":"no"},{"recipe No.":3,"compound":"Resazurin","compound_id":25,"amount":10,"unit":"mg","g_l":0.01,"mmol_l":0.0436319,"optional":"no"},{"recipe No.":4,"compound":"Water","compound_id":754,"condition":"","attribute":"Distilled or deionized","amount":1000,"unit":"ml","optional":"no"}],"main solution":"no"},{"id":1782,"name":"Trace elements solution","volume":1000,"recipe":[{"recipe No.":1,"compound":"Nitrilotriacetic acid","compound_id":189,"attribute":"Aldrich N8407","amount":9.55,"unit":"g","g_l":9.55,"mmol_l":49.9634,"optional":"no"},{"recipe No.":2,"compound":"MgCl2 x 6 H2O","compound_id":79,"amount":4.06,"unit":"g","g_l":4.06,"mmol_l":19.9702,"optional":"no"},{"recipe No.":3,"compound":"FeCl2 x 4 H2O","compound_id":260,"amount":0.98,"unit":"g","g_l":0.98,"mmol_l":4.92928,"optional":"no"},{"recipe No.":4,"compound":"Na2WO4 x 6 H2O","compound_id":803,"amount":0.264,"unit":"g","g_l":0.264,"optional":"no"},{"recipe No.":5,"compound":"NiCl2 x 6 H2O","compound_id":40,"amount":0.118,"unit":"g","g_l":0.118,"mmol_l":0.910501,"optional":"no"},{"recipe No.":6,"compound":"Na2Se2O3","compound_id":804,"amount":0.1,"unit":"g","g_l":0.1,"optional":"no"},{"recipe No.":7,"compound":"Na2MoO4 x 2 H2O","compound_id":9,"amount":0.024,"unit":"g","g_l":0.024,"mmol_l":0.099194,"optional":"no"},{"recipe No.":8,"compound":"CoCl2 x 6 H2O","compound_id":38,"amount":0.023,"unit":"g","g_l":0.023,"mmol_l":0.177142,"optional":"no"},{"recipe No.":9,"compound":"Water","compound_id":754,"condition":"","attribute":"Distilled or deionized","amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Adjust pH of nitrilotriacetic acid in solution to 7.0 with KOH. Add minerals and adjust the final pH to 7.0."}],"main solution":"no"},{"id":1783,"name":" Na2CO3 solution","volume":1000,"recipe":[{"recipe No.":1,"compound":"Na2CO3","compound_id":32,"amount":106,"unit":"g","g_l":106,"mmol_l":1000.11,"optional":"no"},{"recipe No.":2,"compound":"Water","compound_id":754,"attribute":"degassed","amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve 106 g Na2CO3 in 1 liter of degassed H2O. Autoclave in sealed serum bottles under CO2."}],"equipment":{"1204":"Autoclave"},"main solution":"no"},{"id":1784,"name":"Cysteine solutions","volume":50,"recipe":[{"recipe No.":1,"compound":"Water","compound_id":754,"amount":50,"unit":"ml","optional":"no"},{"recipe No.":2,"compound":"L-Cysteine HCl","compound_id":1157,"amount":5,"unit":"g","g_l":100,"mmol_l":634.437,"optional":"no"}],"steps":[{"step":"To 50 ml of boiled and cooled distilled or deionized H2O add 5 g of L-cysteine hydrochloride\r\n. Adjust pH to 10 with 3N NaOH and store under N2 in a serum bottle sealed with a butyl rubber stopper."}],"main solution":"no"},{"id":5663,"name":"Titanium (III) citrate solution (Zehnder and Wuhrmann 1976)","volume":50,"recipe":[{"recipe No.":1,"compound":"Titanium(III) chloride","compound_id":805,"condition":"","attribute":"Merck 808307, 15% solution","amount":5,"unit":"ml","g_l":0.75,"optional":"no"},{"recipe No.":2,"compound":"Sodium citrate","compound_id":792,"condition":"","attribute":"0.2M","amount":50,"unit":"ml","g_l":2.5807,"mmol_l":10,"optional":"no"}],"steps":[{"step":"Neutralize pH with a saturated sodium carbonate solution and store in a serum bottle under N2."}],"main solution":"no"},{"id":5833,"name":"Na2S Solution","volume":50,"recipe":[{"recipe No.":1,"compound":"Water","compound_id":754,"amount":50,"unit":"ml","optional":"no"},{"recipe No.":2,"compound":"Na2S x 9 H2O","compound_id":54,"amount":5,"unit":"g","g_l":100,"mmol_l":416.351,"optional":"no"}],"steps":[{"step":"To 50 ml of boiled and cooled distilled or deionized H2O add 5 g of Na2S x 9 H2O. Adjust pH to 10 with 3N NaOH and store under N2 in a serum bottle sealed with a butyl rubber stopper."}],"main solution":"no"}]}