{"medium":{"id":860,"name":"ANAEROCOLUMNA MEDIUM","complex_medium":"yes","min_pH":7,"max_pH":7.2,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium860.pdf"},"solutions":[{"id":1759,"name":"Main sol. 860","volume":1002,"recipe":[{"recipe No.":1,"compound":"NH4Cl","compound_id":42,"amount":1,"unit":"g","g_l":0.998004,"mmol_l":18.6578,"optional":"no"},{"recipe No.":2,"compound":"K2HPO4","compound_id":10,"amount":0.3,"unit":"g","g_l":0.299401,"mmol_l":1.71896,"optional":"no"},{"recipe No.":3,"compound":"KH2PO4","compound_id":11,"amount":0.3,"unit":"g","g_l":0.299401,"mmol_l":2.2001,"optional":"no"},{"recipe No.":4,"compound":"MgCl2 x 6 H2O","compound_id":79,"amount":0.2,"unit":"g","g_l":0.199601,"mmol_l":0.98179,"optional":"no"},{"recipe No.":5,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":0.1,"unit":"g","g_l":0.0998004,"mmol_l":0.678845,"optional":"no"},{"recipe No.":6,"compound":"KCl","compound_id":61,"amount":0.1,"unit":"g","g_l":0.0998004,"mmol_l":1.3387,"optional":"no"},{"recipe No.":7,"compound":"NaCl","compound_id":43,"amount":0.6,"unit":"g","g_l":0.598802,"mmol_l":10.2464,"optional":"no"},{"recipe No.":8,"compound":"Yeast extract","compound_id":16,"amount":1,"unit":"g","g_l":0.998004,"optional":"no"},{"recipe No.":9,"solution":"Trace element solution SL-10","solution_id":595,"amount":1.5,"unit":"ml","optional":"no"},{"recipe No.":10,"compound":"Sodium resazurin","compound_id":1852,"attribute":"0.1% w/v","amount":0.5,"unit":"ml","g_l":0.000499002,"mmol_l":0.00198671,"optional":"no"},{"recipe No.":11,"compound":"Na2CO3","compound_id":32,"amount":1.5,"unit":"g","g_l":1.49701,"mmol_l":14.1243,"optional":"no"},{"recipe No.":12,"compound":"D-Fructose","compound_id":33,"amount":4,"unit":"g","g_l":3.99202,"mmol_l":22.1582,"optional":"no"},{"recipe No.":13,"compound":"L-Cysteine HCl x H2O","compound_id":770,"amount":0.5,"unit":"g","g_l":0.499002,"mmol_l":2.84111,"optional":"no"},{"recipe No.":14,"compound":"Na2S x 9 H2O","compound_id":54,"amount":0.3,"unit":"g","g_l":0.299401,"mmol_l":1.24656,"optional":"no"},{"recipe No.":15,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve ingredients except carbonate, fructose, cysteine and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add fructose, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 7.0 - 7.2, if necessary."}],"equipment":{"160":"Hungate tubes","3218":"Autoclave","4451":"Gassing station"},"main solution":"yes"},{"id":595,"name":"Trace element solution SL-10","volume":1000,"recipe":[{"recipe No.":1,"compound":"HCl","compound_id":68,"attribute":"25%","amount":10,"unit":"ml","g_l":2.5,"mmol_l":68.5683,"optional":"no"},{"recipe No.":2,"compound":"FeCl2 x 4 H2O","compound_id":260,"amount":1.5,"unit":"g","g_l":1.5,"mmol_l":7.54482,"optional":"no"},{"recipe No.":3,"compound":"ZnCl2","compound_id":64,"amount":70,"unit":"mg","g_l":0.07,"mmol_l":0.513573,"optional":"no"},{"recipe No.":4,"compound":"MnCl2 x 4 H2O","compound_id":36,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.505293,"optional":"no"},{"recipe No.":5,"compound":"H3BO3","compound_id":37,"amount":6,"unit":"mg","g_l":0.006,"mmol_l":0.0970246,"optional":"no"},{"recipe No.":6,"compound":"CoCl2 x 6 H2O","compound_id":38,"amount":190,"unit":"mg","g_l":0.19,"mmol_l":1.46335,"optional":"no"},{"recipe No.":7,"compound":"CuCl2 x 2 H2O","compound_id":39,"amount":2,"unit":"mg","g_l":0.002,"mmol_l":0.0117314,"optional":"no"},{"recipe No.":8,"compound":"NiCl2 x 6 H2O","compound_id":40,"amount":24,"unit":"mg","g_l":0.024,"mmol_l":0.185187,"optional":"no"},{"recipe No.":9,"compound":"Na2MoO4 x 2 H2O","compound_id":9,"amount":36,"unit":"mg","g_l":0.036,"mmol_l":0.148791,"optional":"no"},{"recipe No.":10,"compound":"Distilled water","compound_id":4,"amount":990,"unit":"ml","optional":"no"}],"steps":[{"step":"First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml."}],"Sol. No.":2,"main solution":"no"}]}