{"medium":{"id":827,"name":"THERMOANAEROBACTER SULFUROPHILUS MEDIUM","complex_medium":"yes","min_pH":6.8,"max_pH":7,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium827.pdf"},"solutions":[{"id":1676,"name":"Main sol. 827","volume":1002,"recipe":[{"recipe No.":1,"compound":"NH4Cl","compound_id":42,"amount":0.33,"unit":"g","g_l":0.329341,"mmol_l":6.15706,"optional":"no"},{"recipe No.":2,"compound":"KCl","compound_id":61,"amount":0.33,"unit":"g","g_l":0.329341,"mmol_l":4.41772,"optional":"no"},{"recipe No.":3,"compound":"KH2PO4","compound_id":11,"amount":0.33,"unit":"g","g_l":0.329341,"mmol_l":2.42011,"optional":"no"},{"recipe No.":4,"compound":"MgCl2 x 6 H2O","compound_id":79,"amount":0.33,"unit":"g","g_l":0.329341,"mmol_l":1.61995,"optional":"no"},{"recipe No.":5,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":0.33,"unit":"g","g_l":0.329341,"mmol_l":2.24019,"optional":"no"},{"recipe No.":6,"solution":"Trace element solution SL-10","solution_id":595,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":7,"compound":"Sodium resazurin","compound_id":1852,"attribute":"0.1% w/v","amount":0.5,"unit":"ml","g_l":0.000499002,"mmol_l":0.00198671,"optional":"no"},{"recipe No.":8,"compound":"Sulfur","compound_id":80,"condition":"","attribute":"powdered","amount":10,"unit":"g","g_l":9.98004,"mmol_l":311.195,"optional":"no"},{"recipe No.":9,"compound":"Na2CO3","compound_id":32,"amount":1,"unit":"g","g_l":0.998004,"mmol_l":9.4162,"optional":"no"},{"recipe No.":10,"compound":"Yeast extract","compound_id":16,"amount":0.5,"unit":"g","g_l":0.499002,"optional":"no"},{"recipe No.":11,"compound":"D-Glucose","compound_id":211,"amount":5,"unit":"g","g_l":4.99002,"mmol_l":27.6977,"optional":"no"},{"recipe No.":12,"solution":"Wolin's vitamin solution (10x)","solution_id":5980,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":13,"compound":"Na2S x 9 H2O","compound_id":54,"amount":0.5,"unit":"g","g_l":0.499002,"mmol_l":2.0776,"optional":"no"},{"recipe No.":14,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve ingredients except sulfur, carbonate, yeast extract, glucose, vitamins, and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials containing already the appropriate amount of sulfur. Heat the medium vials to 90-100\u00b0C for 1 hour on each of 3 successive days to sterilize the medium. Add yeast extract, glucose, vitamins (sterilized by filtration), and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use check pH of complete medium and adjust to 6.8 - 7.0, if necessary."}],"equipment":{"941":"Hungate tubes","1990":"Filter","4432":"Gassing station","4701":"Autoclave","4702":"Water bath"},"main solution":"yes"},{"id":595,"name":"Trace element solution SL-10","volume":1000,"recipe":[{"recipe No.":1,"compound":"HCl","compound_id":68,"attribute":"25%","amount":10,"unit":"ml","g_l":2.5,"mmol_l":68.5683,"optional":"no"},{"recipe No.":2,"compound":"FeCl2 x 4 H2O","compound_id":260,"amount":1.5,"unit":"g","g_l":1.5,"mmol_l":7.54482,"optional":"no"},{"recipe No.":3,"compound":"ZnCl2","compound_id":64,"amount":70,"unit":"mg","g_l":0.07,"mmol_l":0.513573,"optional":"no"},{"recipe No.":4,"compound":"MnCl2 x 4 H2O","compound_id":36,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.505293,"optional":"no"},{"recipe No.":5,"compound":"H3BO3","compound_id":37,"amount":6,"unit":"mg","g_l":0.006,"mmol_l":0.0970246,"optional":"no"},{"recipe No.":6,"compound":"CoCl2 x 6 H2O","compound_id":38,"amount":190,"unit":"mg","g_l":0.19,"mmol_l":1.46335,"optional":"no"},{"recipe No.":7,"compound":"CuCl2 x 2 H2O","compound_id":39,"amount":2,"unit":"mg","g_l":0.002,"mmol_l":0.0117314,"optional":"no"},{"recipe No.":8,"compound":"NiCl2 x 6 H2O","compound_id":40,"amount":24,"unit":"mg","g_l":0.024,"mmol_l":0.185187,"optional":"no"},{"recipe No.":9,"compound":"Na2MoO4 x 2 H2O","compound_id":9,"amount":36,"unit":"mg","g_l":0.036,"mmol_l":0.148791,"optional":"no"},{"recipe No.":10,"compound":"Distilled water","compound_id":4,"amount":990,"unit":"ml","optional":"no"}],"steps":[{"step":"First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml."}],"Sol. No.":2,"main solution":"no"},{"id":5980,"name":"Wolin's vitamin solution (10x)","volume":1000,"recipe":[{"recipe No.":1,"compound":"Biotin","compound_id":46,"amount":20,"unit":"mg","g_l":0.02,"mmol_l":0.0818632,"optional":"no"},{"recipe No.":2,"compound":"Folic acid","compound_id":138,"amount":20,"unit":"mg","g_l":0.02,"mmol_l":0.0453104,"optional":"no"},{"recipe No.":3,"compound":"Pyridoxine hydrochloride","compound_id":519,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.486287,"optional":"no"},{"recipe No.":4,"compound":"Thiamine HCl","compound_id":1207,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.148236,"optional":"no"},{"recipe No.":5,"compound":"Riboflavin","compound_id":136,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.132837,"optional":"no"},{"recipe No.":6,"compound":"Nicotinic acid","compound_id":139,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.406141,"optional":"no"},{"recipe No.":7,"compound":"Calcium D-(+)-pantothenate","compound_id":1512,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.209846,"optional":"no"},{"recipe No.":8,"compound":"Vitamin B12","compound_id":18,"amount":1,"unit":"mg","g_l":0.001,"mmol_l":0.00073779,"optional":"no"},{"recipe No.":9,"compound":"p-Aminobenzoic acid","compound_id":47,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.364591,"optional":"no"},{"recipe No.":10,"compound":"(DL)-alpha-Lipoic acid","compound_id":252,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.24233,"optional":"no"},{"recipe No.":11,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"main solution":"no"}]}