{"medium":{"id":825,"name":"METHANOBACTERIUM II MEDIUM","complex_medium":"yes","min_pH":6.8,"max_pH":7,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium825.pdf"},"solutions":[{"id":1673,"name":"Main sol. 825","volume":1011,"recipe":[{"recipe No.":1,"compound":"NH4Cl","compound_id":42,"amount":1,"unit":"g","g_l":0.98912,"mmol_l":18.4917,"optional":"no"},{"recipe No.":2,"compound":"K2HPO4","compound_id":10,"amount":0.3,"unit":"g","g_l":0.296736,"mmol_l":1.70366,"optional":"no"},{"recipe No.":3,"compound":"KH2PO4","compound_id":11,"amount":0.3,"unit":"g","g_l":0.296736,"mmol_l":2.18052,"optional":"no"},{"recipe No.":4,"compound":"MgCl2 x 6 H2O","compound_id":79,"amount":0.2,"unit":"g","g_l":0.197824,"mmol_l":0.97305,"optional":"no"},{"recipe No.":5,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":0.1,"unit":"g","g_l":0.098912,"mmol_l":0.672802,"optional":"no"},{"recipe No.":6,"compound":"KCl","compound_id":61,"amount":0.1,"unit":"g","g_l":0.098912,"mmol_l":1.32679,"optional":"no"},{"recipe No.":7,"compound":"NaCl","compound_id":43,"amount":0.6,"unit":"g","g_l":0.593472,"mmol_l":10.1552,"optional":"no"},{"recipe No.":8,"solution":"Modified Wolin's mineral solution","solution_id":241,"amount":10,"unit":"ml","optional":"no"},{"recipe No.":9,"compound":"Na-acetate","compound_id":23,"amount":0.5,"unit":"g","g_l":0.49456,"mmol_l":6.02873,"optional":"no"},{"recipe No.":10,"compound":"Yeast extract","compound_id":16,"amount":1,"unit":"g","g_l":0.98912,"optional":"no"},{"recipe No.":11,"compound":"Sodium resazurin","compound_id":1852,"attribute":"0.1% w/v","amount":0.5,"unit":"ml","g_l":0.00049456,"mmol_l":0.00196902,"optional":"no"},{"recipe No.":12,"compound":"NaHCO3","compound_id":55,"amount":4,"unit":"g","g_l":3.95648,"mmol_l":47.097,"optional":"no"},{"recipe No.":13,"solution":"Wolin's vitamin solution (10x)","solution_id":5980,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":14,"compound":"L-Cysteine HCl x H2O","compound_id":770,"amount":0.5,"unit":"g","g_l":0.49456,"mmol_l":2.81582,"optional":"no"},{"recipe No.":15,"compound":"Na2S x 9 H2O","compound_id":54,"amount":0.5,"unit":"g","g_l":0.49456,"mmol_l":2.0591,"optional":"no"},{"recipe No.":16,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve ingredients except bicarbonate, vitamins, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve bicarbonate, adjust pH to 7.0, then dispense medium under 80% H2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Add vitamins (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use check pH of complete medium and adjust to 6.8 - 7.0, if necessary."},{"step":"After inoculation pressurize cultivation vessels to 2 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture."}],"equipment":{"746":"Autoclave","2833":"Hungate tubes","3864":"Filter","4429":"Gassing station"},"main solution":"yes"},{"id":241,"name":"Modified Wolin's mineral solution","volume":1000,"recipe":[{"recipe No.":1,"compound":"Nitrilotriacetic acid","compound_id":189,"amount":1.5,"unit":"g","g_l":1.5,"mmol_l":7.84765,"optional":"no"},{"recipe No.":2,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":3,"unit":"g","g_l":3,"mmol_l":12.1716,"optional":"no"},{"recipe No.":3,"compound":"MnSO4 x H2O","compound_id":14,"amount":0.5,"unit":"g","g_l":0.5,"mmol_l":2.9583,"optional":"no"},{"recipe No.":4,"compound":"NaCl","compound_id":43,"amount":1,"unit":"g","g_l":1,"mmol_l":17.1116,"optional":"no"},{"recipe No.":5,"compound":"FeSO4 x 7 H2O","compound_id":12,"amount":0.1,"unit":"g","g_l":0.1,"mmol_l":0.359693,"optional":"no"},{"recipe No.":6,"compound":"CoSO4 x 7 H2O","compound_id":198,"amount":0.18,"unit":"g","g_l":0.18,"mmol_l":1.16132,"optional":"no"},{"recipe No.":7,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":0.1,"unit":"g","g_l":0.1,"mmol_l":0.680203,"optional":"no"},{"recipe No.":8,"compound":"ZnSO4 x 7 H2O","compound_id":35,"amount":0.18,"unit":"g","g_l":0.18,"mmol_l":0.625954,"optional":"no"},{"recipe No.":9,"compound":"CuSO4 x 5 H2O","compound_id":121,"amount":0.01,"unit":"g","g_l":0.01,"mmol_l":0.0400503,"optional":"no"},{"recipe No.":10,"compound":"AlK(SO4)2 x 12 H2O","compound_id":908,"amount":0.02,"unit":"g","g_l":0.02,"mmol_l":0.0421596,"optional":"no"},{"recipe No.":11,"compound":"H3BO3","compound_id":37,"amount":0.01,"unit":"g","g_l":0.01,"mmol_l":0.161708,"optional":"no"},{"recipe No.":12,"compound":"Na2MoO4 x 2 H2O","compound_id":9,"amount":0.01,"unit":"g","g_l":0.01,"mmol_l":0.0413309,"optional":"no"},{"recipe No.":13,"compound":"NiCl2 x 6 H2O","compound_id":40,"amount":0.03,"unit":"g","g_l":0.03,"mmol_l":0.231483,"optional":"no"},{"recipe No.":14,"compound":"Na2SeO3 x 5 H2O","compound_id":107,"amount":0.3,"unit":"mg","g_l":0.0003,"mmol_l":0.00114062,"optional":"no"},{"recipe No.":15,"compound":"Na2WO4 x 2 H2O","compound_id":249,"amount":0.4,"unit":"mg","g_l":0.0004,"mmol_l":0.00121267,"optional":"no"},{"recipe No.":16,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH."}],"Sol. 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