{"medium":{"id":81,"name":"MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3)","complex_medium":"no","min_pH":6.8,"max_pH":6.8,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium81.pdf"},"solutions":[{"id":137,"name":"Main sol. 81","volume":1000,"recipe":[{"recipe No.":1,"solution":"Solution A","solution_id":138,"amount":50,"unit":"ml","optional":"no"},{"recipe No.":2,"solution":"Solution B","solution_id":139,"amount":925,"unit":"ml","optional":"no"},{"recipe No.":3,"solution":"Solution C","solution_id":140,"amount":20,"unit":"ml","optional":"no"},{"recipe No.":4,"solution":"Standard vitamin solution","solution_id":141,"amount":5,"unit":"ml","optional":"no"}],"steps":[{"step":"Solutions A, B, C are autoclaved separately for 15 min at 121\u00b0C, cooled down to 50\u00b0C and then mixed aseptically with 5.0 ml filter-sterilized standard vitamin solution (see below).The final pH of this medium should be 6.8 without adjustment."},{"step":"For chemolithotrophic growth incubate the culture under an atmosphere of 2% (v/v) O2, 10% CO2, 60% H2 and 28% N2. For heterotrophic growth supplement the mineral medium with an appropriate carbon source (0.2% carbohydrate or 0.1% organic acid). For growth on nitrogen-free medium, omit NH4Cl and incubate the culture under an atmosphere of 2% (v/v) O2, 10% CO2, 10% H2 and 78% N2 or heterotrophically under 2% (v/v) O2 and 98% N2. For more details see Ref. 1515 and Ref. 3363."}],"main solution":"yes"},{"id":138,"name":"Solution A","volume":50,"recipe":[{"recipe No.":1,"compound":"KH2PO4","compound_id":11,"amount":2.3,"unit":"g","g_l":46,"mmol_l":338.022,"optional":"no"},{"recipe No.":2,"compound":"Na2HPO4 x 2 H2O","compound_id":181,"amount":2.9,"unit":"g","g_l":58,"mmol_l":408.569,"optional":"no"},{"recipe No.":3,"compound":"Distilled water","compound_id":4,"amount":50,"unit":"ml","optional":"no"}],"main solution":"no"},{"id":139,"name":"Solution B","volume":925,"recipe":[{"recipe No.":1,"compound":"NH4Cl","compound_id":42,"amount":1,"unit":"g","g_l":1.08696,"mmol_l":20.3207,"optional":"no"},{"recipe No.":2,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":0.5,"unit":"g","g_l":0.543478,"mmol_l":2.20499,"optional":"no"},{"recipe No.":3,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":0.01,"unit":"g","g_l":0.0108696,"mmol_l":0.0739351,"optional":"no"},{"recipe No.":4,"compound":"MnCl2 x 4 H2O","compound_id":36,"amount":5,"unit":"mg","g_l":0.00543478,"optional":"no"},{"recipe No.":5,"compound":"NaVO3 x H2O","compound_id":182,"amount":5,"unit":"mg","g_l":0.00543478,"mmol_l":0.0388351,"optional":"no"},{"recipe No.":6,"solution":"Trace element solution SL-6","solution_id":25,"amount":5,"unit":"ml","optional":"no"},{"recipe No.":7,"compound":"Distilled water","compound_id":4,"amount":920,"unit":"ml","optional":"no"},{"recipe No.":8,"compound":"Agar","compound_id":3,"condition":"if required","attribute":"","amount":20,"unit":"g","g_l":21.7391,"mmol_l":64.6363,"optional":"no"}],"main solution":"no"},{"id":140,"name":"Solution C","volume":20,"recipe":[{"recipe No.":1,"compound":"Ferric ammonium citrate","compound_id":97,"amount":0.05,"unit":"g","g_l":2.5,"mmol_l":9.54271,"optional":"no"},{"recipe No.":2,"compound":"Distilled water","compound_id":4,"amount":20,"unit":"ml","optional":"no"}],"equipment":{"1744":"Filter","3736":"Autoclave"},"main solution":"no"},{"id":141,"name":"Standard vitamin solution","volume":100,"recipe":[{"recipe No.":1,"compound":"Riboflavin","compound_id":136,"amount":10,"unit":"mg","g_l":0.1,"mmol_l":0.265675,"optional":"no"},{"recipe No.":2,"compound":"Thiamine-HCl x 2 H2O","compound_id":77,"amount":50,"unit":"mg","g_l":0.5,"mmol_l":1.3394,"optional":"no"},{"recipe No.":3,"compound":"Nicotinic acid","compound_id":139,"amount":50,"unit":"mg","g_l":0.5,"mmol_l":4.06141,"optional":"no"},{"recipe No.":4,"compound":"Pyridoxine hydrochloride","compound_id":519,"amount":50,"unit":"mg","g_l":0.5,"mmol_l":2.43143,"optional":"no"},{"recipe No.":5,"compound":"Calcium pantothenate","compound_id":518,"amount":50,"unit":"mg","g_l":0.5,"mmol_l":1.04925,"optional":"no"},{"recipe No.":6,"compound":"Biotin","compound_id":46,"amount":0.1,"unit":"mg","g_l":0.001,"mmol_l":0.00409316,"optional":"no"},{"recipe No.":7,"compound":"Folic acid","compound_id":138,"amount":0.2,"unit":"mg","g_l":0.002,"mmol_l":0.00453104,"optional":"no"},{"recipe No.":8,"compound":"Vitamin B12","compound_id":18,"amount":1,"unit":"mg","g_l":0.01,"mmol_l":0.0073779,"optional":"no"},{"recipe No.":9,"compound":"Distilled water","compound_id":4,"amount":100,"unit":"ml","optional":"no"}],"main solution":"no"},{"id":25,"name":"Trace element solution SL-6","volume":1000,"recipe":[{"recipe No.":1,"compound":"ZnSO4 x 7 H2O","compound_id":35,"amount":0.1,"unit":"g","g_l":0.1,"mmol_l":0.347752,"optional":"no"},{"recipe No.":2,"compound":"MnCl2 x 4 H2O","compound_id":36,"amount":0.03,"unit":"g","g_l":0.03,"mmol_l":0.151588,"optional":"no"},{"recipe No.":3,"compound":"H3BO3","compound_id":37,"amount":0.3,"unit":"g","g_l":0.3,"mmol_l":4.85123,"optional":"no"},{"recipe No.":4,"compound":"CoCl2 x 6 H2O","compound_id":38,"amount":0.2,"unit":"g","g_l":0.2,"mmol_l":1.54037,"optional":"no"},{"recipe No.":5,"compound":"CuCl2 x 2 H2O","compound_id":39,"amount":0.01,"unit":"g","g_l":0.01,"mmol_l":0.0586569,"optional":"no"},{"recipe No.":6,"compound":"NiCl2 x 6 H2O","compound_id":40,"amount":0.02,"unit":"g","g_l":0.02,"mmol_l":0.154322,"optional":"no"},{"recipe No.":7,"compound":"Na2MoO4 x 2 H2O","compound_id":9,"amount":0.03,"unit":"g","g_l":0.03,"mmol_l":0.123993,"optional":"no"},{"recipe No.":8,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"Sol. No.":1,"main solution":"no"}]}