{"medium":{"id":769,"name":"CLOSTRIDIUM VINCENTII MEDIUM","complex_medium":"yes","min_pH":6.5,"max_pH":6.5,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium769.pdf"},"solutions":[{"id":1567,"name":"Main sol. 769","volume":1001,"recipe":[{"recipe No.":1,"compound":"Sea Salt","compound_id":423,"attribute":"SIGMA","amount":12,"unit":"g","g_l":11.988,"optional":"no"},{"recipe No.":2,"compound":"NH4NO3","compound_id":178,"amount":0.1,"unit":"g","g_l":0.0999001,"optional":"no"},{"recipe No.":3,"compound":"Yeast extract","compound_id":16,"attribute":"BD Bacto","amount":1,"unit":"g","g_l":0.999001,"optional":"no"},{"recipe No.":4,"compound":"Trypticase peptone","compound_id":124,"attribute":"BD BBL","amount":0.4,"unit":"g","g_l":0.3996,"optional":"no"},{"recipe No.":5,"compound":"Sodium resazurin","compound_id":1852,"attribute":"0.1% w/v","amount":0.5,"unit":"ml","g_l":0.0004995,"mmol_l":0.0019887,"optional":"no"},{"recipe No.":6,"compound":"NaHCO3","compound_id":55,"amount":1,"unit":"g","g_l":0.999001,"mmol_l":11.8919,"optional":"no"},{"recipe No.":7,"compound":"Na-phosphate buffer","compound_id":717,"attribute":"0.2 M, pH 6.5","amount":20,"unit":"ml","mmol_l":4,"optional":"no"},{"recipe No.":8,"compound":"Lactose","compound_id":394,"amount":2,"unit":"g","g_l":1.998,"mmol_l":5.83699,"optional":"no"},{"recipe No.":9,"solution":"Wolin's vitamin solution (10x)","solution_id":5980,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":10,"compound":"L-Cysteine HCl x H2O","compound_id":770,"amount":0.3,"unit":"g","g_l":0.2997,"mmol_l":1.70637,"optional":"no"},{"recipe No.":11,"compound":"Na2S x 9 H2O","compound_id":54,"amount":0.3,"unit":"g","g_l":0.2997,"mmol_l":1.2478,"optional":"no"},{"recipe No.":12,"compound":"Distilled water","compound_id":4,"amount":980,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve ingredients (except bicarbonate, phosphate-buffer, lactose, vitamins, cysteine and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add phosphate-buffer, lactose, cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins are prepared under N2 gas atmosphere and sterilized by filtration. Adjust pH of complete medium to 6.5, if necessary."},{"step":"Note: Sea salts can be replaced by 300.0 ml/l of filtered aged sea water."}],"equipment":{"1982":"Autoclave","2786":"Hungate tubes","3809":"Filter","4403":"Gassing station"},"main solution":"yes"},{"id":5980,"name":"Wolin's vitamin solution (10x)","volume":1000,"recipe":[{"recipe No.":1,"compound":"Biotin","compound_id":46,"amount":20,"unit":"mg","g_l":0.02,"mmol_l":0.0818632,"optional":"no"},{"recipe No.":2,"compound":"Folic acid","compound_id":138,"amount":20,"unit":"mg","g_l":0.02,"mmol_l":0.0453104,"optional":"no"},{"recipe No.":3,"compound":"Pyridoxine hydrochloride","compound_id":519,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.486287,"optional":"no"},{"recipe No.":4,"compound":"Thiamine HCl","compound_id":1207,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.148236,"optional":"no"},{"recipe No.":5,"compound":"Riboflavin","compound_id":136,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.132837,"optional":"no"},{"recipe No.":6,"compound":"Nicotinic acid","compound_id":139,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.406141,"optional":"no"},{"recipe No.":7,"compound":"Calcium D-(+)-pantothenate","compound_id":1512,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.209846,"optional":"no"},{"recipe No.":8,"compound":"Vitamin B12","compound_id":18,"amount":1,"unit":"mg","g_l":0.001,"mmol_l":0.00073779,"optional":"no"},{"recipe No.":9,"compound":"p-Aminobenzoic acid","compound_id":47,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.364591,"optional":"no"},{"recipe No.":10,"compound":"(DL)-alpha-Lipoic acid","compound_id":252,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.24233,"optional":"no"},{"recipe No.":11,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"main solution":"no"}]}