{"medium":{"id":76,"name":"GOTTSCHALKIA MEDIUM","complex_medium":"yes","min_pH":7,"max_pH":7.5,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium76.pdf"},"solutions":[{"id":121,"name":"Main sol. 76","volume":1008,"recipe":[{"recipe No.":1,"compound":"KOH","compound_id":126,"amount":0.67,"unit":"g","g_l":0.67,"mmol_l":11.9417,"optional":"no"},{"recipe No.":2,"compound":"K2HPO4","compound_id":10,"amount":0.91,"unit":"g","g_l":0.91,"mmol_l":5.2246,"optional":"no"},{"recipe No.":3,"compound":"Uric acid","compound_id":127,"amount":2,"unit":"g","g_l":2,"mmol_l":11.897,"optional":"no"},{"recipe No.":4,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":0.25,"unit":"g","g_l":0.25,"mmol_l":1.0143,"optional":"no"},{"recipe No.":5,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":15,"unit":"mg","g_l":0.015,"mmol_l":0.10203,"optional":"no"},{"recipe No.":6,"compound":"FeSO4 x 7 H2O","compound_id":12,"attribute":"0.1% w/v in 0.1 N H2SO4","amount":6,"unit":"ml","g_l":0.006,"mmol_l":0.0215816,"optional":"no"},{"recipe No.":7,"solution":"Trace element solution SL-10","solution_id":595,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":8,"solution":"Selenite-tungstate solution","solution_id":777,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":9,"compound":"Yeast extract","compound_id":16,"amount":1,"unit":"g","g_l":1,"optional":"no"},{"recipe No.":10,"compound":"Sodium resazurin","compound_id":1852,"attribute":"0.1% w/v","amount":0.5,"unit":"ml","g_l":0.0005,"optional":"no"},{"recipe No.":11,"compound":"Na2CO3","compound_id":32,"amount":1.5,"unit":"g","g_l":1.5,"mmol_l":14.1525,"optional":"no"},{"recipe No.":12,"compound":"Na-thioglycolate","compound_id":30,"amount":0.5,"unit":"g","g_l":0.5,"mmol_l":4.38216,"optional":"no"},{"recipe No.":13,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"First dissolve KOH and K2HPO4 in water, then add uric acid and boil until the acid is dissolved. Cool medium to room temperature under 100% N2 gas atmosphere and add all other compounds, except carbonate and thioglycolate. Dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave for 15 min at 121\u00b0C. Then add carbonate (filter-sterilized stock solution prepared under 80% N2 and 20% CO2 gas atmosphere) and thioglycolate (stock solution, autoclaved separately under 100% N2 gas). Adjust pH of complete medium to 7.0 - 7.5, if necessary."}],"equipment":{"718":"Hungate tubes","1787":"Filter","3073":"Gassing station","3779":"Autoclave"},"main solution":"yes"},{"id":595,"name":"Trace element solution SL-10","volume":1000,"recipe":[{"recipe No.":1,"compound":"HCl","compound_id":68,"attribute":"25%","amount":10,"unit":"ml","g_l":2.5,"mmol_l":68.5683,"optional":"no"},{"recipe No.":2,"compound":"FeCl2 x 4 H2O","compound_id":260,"amount":1.5,"unit":"g","g_l":1.5,"mmol_l":7.54482,"optional":"no"},{"recipe No.":3,"compound":"ZnCl2","compound_id":64,"amount":70,"unit":"mg","g_l":0.07,"mmol_l":0.513573,"optional":"no"},{"recipe No.":4,"compound":"MnCl2 x 4 H2O","compound_id":36,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.505293,"optional":"no"},{"recipe No.":5,"compound":"H3BO3","compound_id":37,"amount":6,"unit":"mg","g_l":0.006,"mmol_l":0.0970246,"optional":"no"},{"recipe No.":6,"compound":"CoCl2 x 6 H2O","compound_id":38,"amount":190,"unit":"mg","g_l":0.19,"mmol_l":1.46335,"optional":"no"},{"recipe No.":7,"compound":"CuCl2 x 2 H2O","compound_id":39,"amount":2,"unit":"mg","g_l":0.002,"mmol_l":0.0117314,"optional":"no"},{"recipe No.":8,"compound":"NiCl2 x 6 H2O","compound_id":40,"amount":24,"unit":"mg","g_l":0.024,"mmol_l":0.185187,"optional":"no"},{"recipe No.":9,"compound":"Na2MoO4 x 2 H2O","compound_id":9,"amount":36,"unit":"mg","g_l":0.036,"mmol_l":0.148791,"optional":"no"},{"recipe No.":10,"compound":"Distilled water","compound_id":4,"amount":990,"unit":"ml","optional":"no"}],"steps":[{"step":"First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml."}],"Sol. No.":2,"main solution":"no"},{"id":777,"name":"Selenite-tungstate solution","volume":1000,"recipe":[{"recipe No.":1,"compound":"NaOH","compound_id":256,"amount":0.5,"unit":"g","g_l":0.5,"mmol_l":12.5009,"optional":"no"},{"recipe No.":2,"compound":"Na2SeO3 x 5 H2O","compound_id":107,"amount":3,"unit":"mg","g_l":0.003,"mmol_l":0.0114062,"optional":"no"},{"recipe No.":3,"compound":"Na2WO4 x 2 H2O","compound_id":249,"amount":4,"unit":"mg","g_l":0.004,"mmol_l":0.0121267,"optional":"no"},{"recipe No.":4,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"Sol. No.":3,"main solution":"no"}]}