{"medium":{"id":63,"name":"DESULFOVIBRIO (POSTGATE) MEDIUM","complex_medium":"yes","min_pH":6.8,"max_pH":7,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium63.pdf"},"solutions":[{"id":90,"name":"Main sol. 63","volume":1000,"recipe":[{"recipe No.":1,"solution":"Solution A","solution_id":91,"amount":980,"unit":"ml","optional":"no"},{"recipe No.":2,"solution":"Solution B","solution_id":92,"amount":10,"unit":"ml","optional":"no"},{"recipe No.":3,"solution":"Solution C","solution_id":93,"amount":10,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve ingredients of solution A, bring to the boil, then cool to room temperature while sparging with 100% N2 gas. Add solutions B and C, adjust pH to 7.8 with NaOH, and distribute under 100% N2 gas atmosphere into anoxic Hungate-type tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended. Autoclave 15 min at 121 \u00b0C. Adjust pH of the complete medium to 6.8 - 7.0, if necessary."}],"equipment":{"3937":"Autoclave","3938":"Hungate tubes","3949":"Gassing station"},"main solution":"yes"},{"id":91,"name":"Solution A","volume":980,"recipe":[{"recipe No.":1,"compound":"K2HPO4","compound_id":10,"amount":0.5,"unit":"g","g_l":0.510204,"mmol_l":2.92924,"optional":"no"},{"recipe No.":2,"compound":"NH4Cl","compound_id":42,"amount":1,"unit":"g","g_l":1.02041,"mmol_l":19.0766,"optional":"no"},{"recipe No.":3,"compound":"Na2SO4","compound_id":110,"amount":1,"unit":"g","g_l":1.02041,"mmol_l":7.18395,"optional":"no"},{"recipe No.":4,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":0.1,"unit":"g","g_l":0.102041,"mmol_l":0.694084,"optional":"no"},{"recipe No.":5,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":2,"unit":"g","g_l":2.04082,"mmol_l":8.27998,"optional":"no"},{"recipe No.":6,"compound":"Na-DL-lactate","compound_id":111,"amount":2,"unit":"g","g_l":2.04082,"mmol_l":18.2118,"optional":"no"},{"recipe No.":7,"compound":"Yeast extract","compound_id":16,"amount":1,"unit":"g","g_l":1.02041,"optional":"no"},{"recipe No.":8,"compound":"Sodium resazurin","compound_id":1852,"attribute":"0.1% w/v","amount":0.5,"unit":"ml","g_l":0.000510204,"mmol_l":0.00203131,"optional":"no"},{"recipe No.":9,"compound":"Distilled water","compound_id":4,"amount":980,"unit":"ml","optional":"no"}],"main solution":"no"},{"id":92,"name":"Solution B","volume":10,"recipe":[{"recipe No.":1,"compound":"FeSO4 x 7 H2O","compound_id":12,"amount":0.5,"unit":"g","g_l":50,"mmol_l":179.846,"optional":"no"},{"recipe No.":2,"compound":"Distilled water","compound_id":4,"amount":10,"unit":"ml","optional":"no"}],"main solution":"no"},{"id":93,"name":"Solution C","volume":10,"recipe":[{"recipe No.":1,"compound":"Na-thioglycolate","compound_id":30,"amount":0.1,"unit":"g","g_l":10,"mmol_l":87.6432,"optional":"no"},{"recipe No.":2,"compound":"Ascorbic acid","compound_id":112,"amount":0.1,"unit":"g","g_l":10,"mmol_l":56.7795,"optional":"no"},{"recipe No.":3,"compound":"Distilled water","compound_id":4,"amount":10,"unit":"ml","optional":"no"}],"equipment":{"397":"Hungate tubes","3457":"Autoclave"},"main solution":"no"}]}