{"medium":{"id":590,"name":"DICHOTOMICROBIUM THERMOHALOPHILUM MEDIUM","complex_medium":"yes","min_pH":7,"max_pH":7.2,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium590.pdf"},"solutions":[{"id":1266,"name":"Main sol. 590","volume":1000,"recipe":[{"recipe No.":1,"compound":"Yeast extract","compound_id":16,"attribute":"Difco, Bacto ","amount":1,"unit":"g","g_l":1,"optional":"no"},{"recipe No.":2,"compound":"Na2-DL-malate","compound_id":204,"amount":1,"unit":"g","g_l":1,"mmol_l":5.61637,"optional":"no"},{"recipe No.":3,"solution":"Hutner's salts","solution_id":1268,"amount":20,"unit":"ml","optional":"no"},{"recipe No.":4,"solution":"Artificial sea water, 3 times concentrated","solution_id":1267,"condition":"","attribute":"","amount":980,"unit":"ml","optional":"no"},{"recipe No.":5,"compound":"Agar","compound_id":3,"condition":"for solid medium","amount":18,"unit":"g/l","g_l":18,"mmol_l":53.5189,"optional":"no"},{"recipe No.":6,"compound":"NaHCO3","compound_id":55,"attribute":"8 %","amount":28.4,"unit":"ml","g_l":2.272,"mmol_l":27.0454,"optional":"no"}],"steps":[{"step":"pH 7.0-7.2"},{"step":"Agar at a concentration of 18 g/l is added for solid media."},{"step":"Prepare an 8% solution of NaHCO3 in half full 100ml serum bottles closed with a rubber\r\nseptum and autoclave. Add 28.4 ml of the sterile 8% solution of NaHCO3 (2.822g/l) per\r\nlitre of the sterile, cooled medium."}],"main solution":"yes"},{"id":1267,"name":"Artificial sea water, 3 times concentrated","volume":1000,"recipe":[{"recipe No.":1,"compound":"NaCl","compound_id":43,"amount":70.43,"unit":"g","g_l":70.43,"mmol_l":1205.17,"optional":"no"},{"recipe No.":2,"compound":"Na2SO4","compound_id":110,"amount":11.75,"unit":"g","g_l":11.75,"mmol_l":82.7232,"optional":"no"},{"recipe No.":3,"compound":"NaHCO3","compound_id":55,"amount":2.88,"unit":"g","g_l":2.88,"mmol_l":34.2829,"optional":"no"},{"recipe No.":4,"compound":"KCl","compound_id":61,"amount":1.99,"unit":"g","g_l":1.99,"mmol_l":26.6935,"optional":"no"},{"recipe No.":5,"compound":"KBr","compound_id":174,"amount":0.29,"unit":"g","g_l":0.29,"mmol_l":2.43697,"optional":"no"},{"recipe No.":6,"compound":"MgCl2 x 6 H2O","compound_id":79,"amount":31.86,"unit":"g","g_l":31.86,"mmol_l":156.712,"optional":"no"},{"recipe No.":7,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":4.35,"unit":"g","g_l":4.35,"mmol_l":29.5888,"optional":"no"},{"recipe No.":8,"compound":"H3BO3","compound_id":37,"amount":0.08,"unit":"g","g_l":0.08,"mmol_l":1.29366,"optional":"no"},{"recipe No.":9,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Add NaHCO3 from a filter-sterilized stock solution when the medium has cooled."}],"equipment":{"939":"Filter"},"main solution":"no"},{"id":1268,"name":"Hutner's salts","volume":1000,"recipe":[{"recipe No.":1,"compound":"Nitrilotriacetic acid","compound_id":189,"attribute":"NTA","amount":10,"unit":"g","g_l":10,"mmol_l":52.3177,"optional":"no"},{"recipe No.":2,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":29.7,"unit":"g","g_l":29.7,"mmol_l":120.499,"optional":"no"},{"recipe No.":3,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":3.335,"unit":"g","g_l":3.335,"mmol_l":22.6848,"optional":"no"},{"recipe No.":4,"compound":"(NH4)6Mo7O24 x 4 H2O","compound_id":120,"amount":9.25,"unit":"mg","g_l":0.00925,"mmol_l":0.00748467,"optional":"no"},{"recipe No.":5,"compound":"FeSO4 x 7 H2O","compound_id":12,"amount":99,"unit":"mg","g_l":0.099,"mmol_l":0.356096,"optional":"no"},{"recipe No.":6,"solution":"\"Metals 44\"","solution_id":1269,"amount":50,"unit":"ml","optional":"no"},{"recipe No.":7,"compound":"Distilled water","compound_id":4,"amount":950,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve the nitrilotriacetic acid."},{"step":"Adjust pH to 7.0 with KOH (~7.3 g)."},{"step":"Dissolve other salts separately and combine."},{"step":"Adjust pH to 6.8 with NaOH or H2SO4."}],"Sol. No.":32,"main solution":"no"},{"id":1269,"name":"\"Metals 44\"","volume":1000,"recipe":[{"recipe No.":1,"compound":"Na-EDTA","compound_id":589,"amount":250,"unit":"mg","g_l":0.25,"mmol_l":0.743592,"optional":"no"},{"recipe No.":2,"compound":"ZnSO4 x 7 H2O","compound_id":35,"amount":1095,"unit":"mg","g_l":1.095,"mmol_l":3.80789,"optional":"no"},{"recipe No.":3,"compound":"FeSO4 x 7 H2O","compound_id":12,"amount":500,"unit":"mg","g_l":0.5,"mmol_l":1.79846,"optional":"no"},{"recipe No.":4,"compound":"MnSO4 x H2O","compound_id":14,"amount":154,"unit":"mg","g_l":0.154,"mmol_l":0.911156,"optional":"no"},{"recipe No.":5,"compound":"CuSO4 x 5 H2O","compound_id":121,"amount":39.2,"unit":"mg","g_l":0.0392,"mmol_l":0.156997,"optional":"no"},{"recipe No.":6,"compound":"Co(NO3)2 x 6 H2O","compound_id":541,"amount":24.8,"unit":"mg","g_l":0.0248,"mmol_l":0.0852131,"optional":"no"},{"recipe No.":7,"compound":"Na2B4O7 x 10 H2O","compound_id":196,"amount":17.7,"unit":"mg","g_l":0.0177,"optional":"no"},{"recipe No.":8,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions."}],"Sol. No.":46,"main solution":"no"}]}