{"medium":{"id":585,"name":"BCYE AGAR","complex_medium":"yes","source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium585.pdf"},"solutions":[{"id":1250,"name":"Main sol. 585","volume":1000,"recipe":[{"recipe No.":1,"compound":"OXOID Legionella CYE-Agar base","compound_id":2125,"attribute":"with Legionella BCYE supplement","amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Dehydrated media preparations are available from media distributors."},{"step":"We use OXOID Legionella CYE-Agar base with Legionella BCYE supplement."},{"step":"For solubilization of the lyophilized pellet use standard liquid media. Although Legionellae do not grow in those media they are suitable for resuspension and transfer onto agar plates."},{"step":"Note: Alternatively use CYE-ACES AGAR"}],"main solution":"yes"},{"id":1252,"name":"CYE-ACES AGAR","volume":1000,"recipe":[{"recipe No.":1,"solution":"Solution A","solution_id":1253,"amount":490,"unit":"ml","optional":"no"},{"recipe No.":2,"solution":"Solution B","solution_id":1254,"amount":490,"unit":"ml","optional":"no"},{"recipe No.":3,"solution":"Solution C","solution_id":1255,"amount":10,"unit":"ml","optional":"no"},{"recipe No.":4,"solution":"Solution D","solution_id":1256,"amount":10,"unit":"ml","optional":"no"}],"steps":[{"step":"Add Cysteine-HCl, then ferric pyrophosphate solution to solution A. Adjust the complete medium to pH 6.9 \u00b1 0.5 at 50\u00b0C with sterile 1.0 N KOH or HCl. The pH of the medium is critical. Finally, add solution B. Swirl medium in flask during dispensing to petri dishes or tubes in order to keep charcoal suspended."}],"main solution":"no"},{"id":1253,"name":"Solution A","volume":490,"recipe":[{"recipe No.":1,"compound":"Yeast extract","compound_id":16,"attribute":"Difco 0127","amount":10,"unit":"g","g_l":20.4082,"optional":"no"},{"recipe No.":2,"compound":"ACES","compound_id":581,"attribute":"N-2-acetamido-2-aminoethane-sulfonic acid","amount":10,"unit":"g","g_l":20.4082,"mmol_l":112.01,"optional":"no"},{"recipe No.":3,"compound":"Activated charcoal","compound_id":582,"attribute":"Sigma C 5510","amount":2,"unit":"g","g_l":4.08163,"optional":"no"},{"recipe No.":4,"compound":"Distilled water","compound_id":4,"amount":490,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve ACES and bring to pH 6.9 by slowly adding 1 N KOH. Add the other components and dissolve by boiling. Autoclave at 121\u00b0C for 15 minutes. Cool to 50 - 55\u00b0C."}],"equipment":{"1910":"Autoclave"},"main solution":"no"},{"id":1254,"name":"Solution B","volume":490,"recipe":[{"recipe No.":1,"compound":"Agar","compound_id":3,"amount":15,"unit":"g","g_l":30.6122,"optional":"no"},{"recipe No.":2,"compound":"Distilled water","compound_id":4,"amount":490,"unit":"ml","optional":"no"}],"steps":[{"step":"Autoclave at 121\u00b0C and cool to 50 - 55\u00b0C."}],"equipment":{"3172":"Autoclave"},"main solution":"no"},{"id":1255,"name":"Solution C","volume":10,"recipe":[{"recipe No.":1,"compound":"L-Cysteine HCl x H2O","compound_id":770,"amount":0.4,"unit":"g","g_l":40,"mmol_l":227.744,"optional":"no"},{"recipe No.":2,"compound":"Distilled water","compound_id":4,"amount":10,"unit":"ml","optional":"no"}],"steps":[{"step":"Prepare a fresh solution and filter-sterilize it."}],"equipment":{"3430":"Filter"},"main solution":"no"},{"id":1256,"name":"Solution D","volume":10,"recipe":[{"recipe No.":1,"compound":"Fe4(PO4)2","compound_id":583,"amount":0.25,"unit":"g","g_l":25,"mmol_l":33.5475,"optional":"no"},{"recipe No.":2,"compound":"Distilled water","compound_id":4,"amount":10,"unit":"ml","optional":"no"}],"steps":[{"step":"Prepare a fresh solution by heating to 50 - 55\u00b0C in a water-bath. Filter-sterilize solution separately."},{"step":"Please note: Soluble ferric pyrophosphate must be kept dry and in the dark. Do not use if chemical looses its green colour and becomes brown or yellow."}],"equipment":{"3829":"Filter"},"main solution":"no"}]}