{"medium":{"id":"535b","name":"REACTIVATION WITH LIQUID MEDIUM","complex_medium":"yes","min_pH":7.3,"max_pH":7.3,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium535b.pdf"},"solutions":[{"id":1183,"name":"Main sol. 535b","volume":1000,"recipe":[{"recipe No.":1,"solution":"Main sol. 535","solution_id":1181,"condition":"alternative","amount":1000,"unit":"ml","optional":"no"},{"recipe No.":2,"solution":"545. TRYPTONE SOYA BROTH (TSB)","solution_id":1185,"condition":"alternative","amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 545. Subsequent subculturing may be carried out in liquid medium 545 or on agar plates."}],"main solution":"yes"},{"id":1185,"name":"545. TRYPTONE SOYA BROTH (TSB)","volume":1000,"recipe":[{"recipe No.":1,"compound":"Casein peptone","compound_id":21,"amount":17,"unit":"g","g_l":17,"optional":"no"},{"recipe No.":2,"compound":"Soy peptone","compound_id":75,"amount":3,"unit":"g","g_l":3,"optional":"no"},{"recipe No.":3,"compound":"D(+)-Glucose","compound_id":94,"amount":2.5,"unit":"g","g_l":2.5,"mmol_l":13.8766,"optional":"no"},{"recipe No.":4,"compound":"NaCl","compound_id":43,"amount":5,"unit":"g","g_l":5,"mmol_l":85.5578,"optional":"no"},{"recipe No.":5,"compound":"K2HPO4","compound_id":10,"amount":2.5,"unit":"g","g_l":2.5,"mmol_l":14.3533,"optional":"no"},{"recipe No.":6,"compound":"Agar","compound_id":3,"amount":15,"unit":"g","g_l":15,"mmol_l":44.5991,"optional":"no"},{"recipe No.":7,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Adjust pH to 7.3."},{"step":"Medium 545 is the same as Caso Bouillon (Merck or Oxoid, Germany)."}],"main solution":"no"},{"id":1181,"name":"Main sol. 535","volume":1000,"recipe":[{"recipe No.":1,"compound":"Trypticase soy broth","compound_id":99,"attribute":"BBL 11768, Oxoid CM129 or Merck 5459","amount":30,"unit":"g","optional":"no"},{"recipe No.":2,"compound":"Agar","compound_id":3,"amount":20,"unit":"g","optional":"no"},{"recipe No.":3,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"pH 7.3"},{"step":"Autoclave at 121\u00b0C for 15 min."}],"equipment":{"2366":"Autoclave"},"main solution":"no"}]}